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人巨噬细胞集落刺激因子受体ELISA Kit价格/M-CSFR ELISA试剂盒说明书
点击次数:88发布时间:2015/11/11 15:27:36
更新日期:2015/11/11 15:27:36
所 在 地:中国大陆
产品型号:48T/96T
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详细内容
Human Macrophage Colony-Stimulating Factor Receptor,M-CSFR ELISA Kit
实验原理
用纯化的抗原包被微孔板,制成固相载体,往包被抗原的微孔中依次加入标本或标准品、生物素化的抗原、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的指标呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样品浓度。
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