环指蛋白135抗体适用哪些实验

中文名称：环指蛋白135抗体
别       名：L13; MGC13061; ring finger protein 135; RN135_HUMAN . 
研究领域：神经生物学  转录调节因子  表观遗传学  环指蛋白 
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human, Mouse, Rat, Dog, Cow,
产品应用：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量：48kDa
细胞定位：细胞浆
性    状：Liquid
浓    度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived from human RNF135:288-360/432
亚    型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍：The RING-type zinc finger motif is present in a number of viral ａnd eukaryotic proteins ａnd is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF135 (RING finger protein 135), also known as L13, is a 432 amino acid protein that contains one RING-type zinc finger ａnd one SPRY domain. Via its RING-type zinc finger, RNF135 may play a role in transcriptional regulation ａnd protein degradation events. Defects in the gene encoding RNF135 are the cause of RNF135-related overgrowth syndrome which is characterized by learning disabilities, facial dysmorphism ａnd increased weight ａnd height. Multiple isoforms of RNF135 exist due to alternative splicing events.

Function:
The protein encoded by RNF135 contains a RING finger domain, a motif present in a variety of functionally distinct proteins ａnd known to be involved in protein-protein ａnd protein-DNA interactions. This gene is located in a chromosomal region known to be frequently deleted in patients with neurofibromatosis.

Subunit:
Interacts with DDX58. Interacts with PCBP2.

Subcellular Location:
Cytoplasmic

Tissue Specificity:
Expressed in skeletal muscle, spleen, kidney,placenta, prostate, stomach, thyroid ａnd tongue. Also weaklyexpressed in heart, thymus, liver ａnd lung.

Post-translational modifications:
Defects in RNF135 are the cause of macrocephalymacrosomia facial dysmorphism syndrome (MMFD) [MIM:614192]. MMFD isan autosomal dominant disorder characterized by the association ofmacrothrombocytopathy ａnd progressive sensorineural hearing losswithout renal dysfunction.