艾美捷髓过氧化物酶MPO（人）ELISA试剂盒检测原理：

原理 人血清蛋白酶联免疫吸附试验是一种基于三明治原理的即用型固相酶联免疫吸附试验，工作时间为3个半小时。由12个一次性8孔条组成的板块的有效形式允许自由选择检测的批量大小。样品和标准品在涂有识别人MPO的抗体的微孔中孵化。生物素化的示踪剂抗体将与捕获的人MPO结合。链霉亲和素-过氧化物酶结合物将与生物素化示踪抗体结合。链霉蛋白-过氧化物酶结合物将与底物四甲基联苯胺（TMB）反应。酶的反应通过加入草酸停止。用分光光度计测量450纳米处的吸光度。通过绘制吸光度（线性）与人类MPO标准品的相应浓度（对数）的关系，得到标准曲线。与标准品同时进行的样品的人类MPO浓度可由标准曲线确定。

艾美捷髓过氧化物酶MPO（人）ELISA试剂盒参考文献：

1. Leclercq A et al; Involvement of intraplaque hemorrhage in atherothombosis evolution via neutrophil protease enrichment. J Leukoc Biol 2007, 82: 1420

2. Martin-Ventura J et al ; Low plasma levels of HSP70 in patients with carotid atherosclerosis are associated with increased levels of proteolytic markers of neutrophil activation. Atherosclerosis 2007, 194: 334

3. Puklo, M et al; Analysis of neutrophil-derived antimicrobial peptides in gingival crevicular fluid suggests importance of cathelicidin LL-37 in the innate immune response against periodontogenic bacteria. Oral Microbiol Immunol 2008, 23: 328

4. Haan de, J et al; Postshock intervention with high-lipid enteral nutrition reduces inflammation ａnd tissue damage. Ann Surg 2008, 248: 842

艾美捷髓过氧化物酶MPO（人）ELISA试剂盒相关研究：

MPO, Mouse, ELISA kit

MPO, Rat, ELISA kit

MPO, Human, ELISA kit

MPO, Human, mAb 266-6K1, biotinylated

MPO, Rat, mAb 2D4, biotinylated

MPO, Human, pAb

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