The RMP1-14-CP151 monoclonal antibody is a chimeric version of the original RMP1-14 antibody. The variable domain sequences are identical to the original RMP1-14 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The RMP1-14-CP151 antibody also contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. RMP1-14-CP151 reacts with mouse PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 ａnd CD8 thymocytes as well as activated T ａnd B lymphocytes ａnd myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 ａnd PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, ａnd T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance ａnd prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, ａnd loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, ａnd breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 ａnd its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments.

BioXCell RecombiMAb小鼠PD-1(CD279)抗体(D265A)：

RMP1-14-CP151单克隆抗体是原始RMP1-14抗体的嵌合版本。可变结构域序列与原始RMP1-14相同，但恒定区域序列已从大鼠IgG2a切换到小鼠IgG2a。RMP1-14-CP151抗体在Fc片段中还含有D265A突变，使其无法与内源性Fcγ受体结合。RMP1-14-CP151与小鼠PD-1（程序性死亡-1）反应，也称为CD279。PD-1是由Pdcd50基因编码的55-1 kDa细胞表面受体，属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上短暂表达。PD-1在成功消除抗原后表达下降。

BioXCell艾美捷RecombiMAb小鼠PD-1(CD279)抗体(D265A)规格：

#  BXC-CP151

Isotype Mouse IgG2a, κ

Recommended Isotype Control(s) RecombiMAb mouse IgG2a (D265A) isotype control, anti-hen egg lysozyme

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Mutations D265A

Immunogen Syrian Hamster BKH cells transfected with mouse PD-1 cDNA

Reported Applications in vivo blocking of PD-1/PD-L signaling*

*Reported for the original rat IgG2a RMP1-14 antibody

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <1EU/mg (<0.001EU/μg)

Determined by LAL gel clotting assay

Aggregation <5%

Determined by DLS

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from CHO cell supernatant in an animal free facility

Purification Protein G

RRID AB_2927525

Molecular Weight 150 kDa

Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同种型 小鼠IgG2a， κ

推荐的同种型对照 RecombiMAb小鼠IgG2a（D265A）同种型对照，抗母蛋溶菌酶

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

突变 D265A

免疫原 用小鼠PD-1 cDNA转染的叙利亚仓鼠BKH细胞

报告的应用程序 体内阻断PD-1/PD-L信号传导**

报道了原始大鼠IgG2a RMP1-14抗体

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <1EU/mg （<0.001EU/μg）

通过LAL凝胶凝血测定法测定

集合体 <5%

由 DLS 确定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μM 过滤

生产 从无动物设施中的CHO细胞上清液中纯化

纯化 蛋白 G

里德 AB_2927525

分子量 150 千达

鼠病原体检测 外切粒菌/鼠痘病毒： 阴性 汉坦病毒： 阴性 K 病毒： 阴性乳酸脱氢酶升高病毒： 阴性 淋巴细胞性脉络丛脑膜炎病毒： 阴性小鼠腺病毒： 阴性小鼠巨细胞病毒： 阴性小鼠肝炎病毒： 阴性小鼠分钟病毒： 阴性小鼠诺如病毒： 阴性小鼠细小病毒： 阴性

小鼠轮状病毒：肺支原体阴性：阴性 小鼠肺炎病毒：阴性 多瘤病毒：阴性呼肠孤病毒筛查：阴性 仙台病毒：阴性 泰勒鼠脑脊髓炎：阴性

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

来源：https://www.amyjet.com/products/BXC-CP151-1mg.shtml