Blue-dextrans are synthesized from controlled dextran fractions by reacting with Cibacron blue F3GA. After purification from non-bound dye, the products are controlled for mean weight average molecular weight (Mw), solubility, degree of substitution, free dye ａnd loss on drying. The low molecular weight blue-dextrans (BD5, BD10 ａnd BD20) require purification by chromatographic methods during production ａnd are therefore more expensive than the larger ones.

The products are designated by the approximate molecular weights of the dextran fractions used. Thus, for example, the product BD5 has a molecular weight of approx. 5000. The actual molecular weight is determined by gel permeation chromatography (GPC). This value is supplied with the Certificate of Analysis. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear α-(1-6)-linked glucose chain with a low percentage (2-5%) of α-(1-3) branches distributed along the chain. The dextran fractions used are from weight average molecular weights (Mw) of 4000 to 2000000 ａnd are carefully controlled by GPC, optical rotation, absorbance ａnd other control parameters

蓝色葡聚糖2000长期以来一直被用作色谱法中的空隙体积标记物，并且用于色谱 法的蓝色-葡聚糖凝胶缀合物已经使用多年（例如参见GE Healthcare Life Sciences；分子排阻色谱法；原理和方法）。使用蓝色葡聚糖的其它重要研究域包括：

溶酶体活性的研究

内皮细胞通透性

牛精子渗透性

角膜通透性

肺血流研究

脑通透性

蛋白质和酶与蓝色葡聚糖的结合

蓝色葡聚糖是通过与Cibacron蓝F3GA反应由受控右旋糖聚糖部分合成的。从非结合染料中纯化后，对产物的平均重量进行控制分子量（Mw）、溶解度、取代度、游离染料和干燥损失。低点分子量蓝色蓝色葡聚糖（BD5、BD10和BD20）需要通过色谱纯化方法，因此比较大的方法更昂贵。产品由所用葡聚糖部分的近似分子量指定。

因此，例如，产物BD5具有大约5000的分子量。实际分子通过凝胶渗透色谱法（GPC）测定重量。此值随分析证书。所使用的葡聚糖来自间质明串珠菌B-512F基本上是线性α-（1-6）连接的葡萄糖链，具有低百分比（2-5%）的α-（1-3）分支沿着链条分布。所使用的葡聚糖组分来自重均分子量（Mw）为4000至2000000，并通过GPC、旋光度、吸光度和其他仔细控制控制参数。

艾美捷Blue dextran（蓝色葡聚糖）#TDB-BD5、BD10和BD20：是一种蓝色无味粉末，可自由溶解于水或电解质溶液中。由于存在两种磺酸盐，该产品具有明显的两性特征以及Cibacron Blue中的氨基。由于这种性质可能会影响溶液中的流体动力学体积以及与凝胶的相互作用，因此在GPC上运行样品时发现了一些异常。

蓝色葡聚糖不溶于大多数有机溶剂，例如乙醇、甲醇、丙酮，氯丨仿、乙酸乙酯等。蓝色葡聚糖110在水中的吸收光谱所使用的发色团在621.5nm处具有大吸光度。

文献参考：

1. D.M.Wolfe, J.H.Lee, A.Kumar et al., Auhagy failure in Alzheimers disease ａnd role of defective lysosomal acidification, Eur.J.Neurosci., 2013,37(12), 1949-61.

2. C.M.Waters, J.S.Alexander, T.R.Harris et al., Perilla ketone increases endothelial cell monlayer in vitro, J.Appl.Physiol., 1993,74(5), 24932501

3. P.K.Schoff ａnd N.L.First, Stimulation of bovine cell motility by the triazine dye cibacron blue F3GA, Mol.Reprod.dev., 1995,42(1), 65-71.

4. R.M.Robbins ａnd M.A.Galin, A model for steroid effects in herpes keratitis, Arch.Ophthalmol., 1975,93(9),828-30.

5. S.T.Ballard ａnd J.T.Gatzy, Volume flow across the alveolar epithelium of adult rat lung, J.Appl.Physiol., 1991,70(4),1665-16676.

6. S.Cassin, Effect of indomethacin on fetal lung liquid formation, Can.J.Physiol.Pharmacol., 1984,62(1),157-9

7. R.E.Pfister, C.A.Ramsden, H.L.Neil et al., Errors in estimating lung liquid in fetal lambs when using radiolabelled serum albumin ａnd blue dextran, J.Appl.Physiol., 1999, 87(6) 2366-74

8. C.E.Johanson, Age-related decrease inA-Beta Peptide clearance pathways: CSF ａnd BBB, RePORTER (Database NIH),

9. S.S.Prabhu, Broaddus, G.T.Gillies et al., Distribution of macromolecular dyes in brain using positive pressure. Surg.Neurol., 1998, 50(40) 367-375.

10. L.Z.Bito, H.Davidson ａnd J.R.Hollingsworth, Facilitated transport of prostaglandins across the blood-cerebrospinal fluid an blood-brain barrier, J.Physiol., 1976, 256(2), 273-85

11. Reshma SV1, Sathyanarayanan N, Nagendra HG, Characterization of hypothetical protein VNG0128C from Halobacterium NRC-1 reveals GALE like activity ａnd its involvement in Leloir pathway of galactose metabolism, J Biomol Struct Dyn. 2014 Nov 14:1-13.

12. G.E.J.Staal, J.F.Koster, H.Kamp et al., Human erythrocyte pyruvate kinase. Its purification ａnd some properties, Biochim.Biophys.Acta, 1971, 227, 86-89

13. A.C.W.Swart ａnd H.C.Hemker, Separation of blood coagulation factors II,VII,IX ａnd X by gel filtration in the presence of Dextran blue, Biochim.Biophys.Acta, 1970, 222, 692-695

来源：https://www.amyjet.com/brand/blue-dextran.shtml