Magnetic nanoparticles are made of completely biodegradable iron oxide ａnd coated with specific proprietary cationic molecules, with different applications. Their binding to gene vectors (DNA, siRNA, ODN, viruses, etc.) is achieved through salt induced colloidal aggregation ａnd electrostatic interactions. Then, under the influence of an external magnetic field generated by a specific magnetic plate, magnetic particles are concentrated on the cell. The uptake of genetic material by cells is accomplished through two natural biological processes, endocytosis ａnd pinocytosis. Therefore, compared to other physical transfection methods that destroy, generate pores, or shock cell membranes, the membrane structure remains intact. Then, according to the formula used, nucleic acids are released into the cylasm through different mechanisms. The first is the proton sponge effect caused by cationic polymers coated on nanoparticles, which promotes the osmotic expansion of the endosomes, damages the membrane of the endosomes, ａnd releases DNA within cells. The second is the destruction of cationic lipids enveloping the endosomes on particles, which release nucleic acids into the cell through the flipping ａnd charge neutralization of negative lipids in the cell. The third is the common virus mechanisms during virus use.

艾美捷Mega Magnetic Plate超大磁性板（MF14000）可同时容纳4个培养皿或培养皿。

OZ Biosciences磁性板适用于所有磁感染试剂和所有细胞培养皿（384、96、4、24、12、6孔板、35、60、90和100 mm培养皿、T-25、T-75和任何其他烧瓶）可以在孵化器和机器人中使用刻在磁性板上的OZ生物科学指示要执行的一侧磁性感染。笔记。磁转染™ 技术需要适当的磁场来磁化纳米颗粒在溶液中，形成一个非常强的梯度来吸引纳米颗粒，并覆盖所有板的表面。

该磁性转染（Magnetofection）原理是将核酸、转染试剂或病毒与特异性阳离子磁性纳米颗粒结合。然后浓缩所得的分子复合物并转运到由适当磁场支持的细胞中。通过这种方式，利用施加在基因载体上的磁力，可以非常快速地将整个载体剂量集中吸引到细胞上，使100%的细胞接触到显著的载体剂量，并促进细胞摄取。

磁性纳米颗粒由完全可生物降解的氧化铁制成，涂有特定的有阳离子分子，应用不同。它们与基因载体（DNA、siRNA、ODN、病毒等）的结合是通过盐诱导的胶体聚集和静电相互作用实现的。然后，通过由特定磁性板产生的外部磁场的影响，磁性颗粒集中在细胞上。细胞对遗传物质的摄取是通过内吞作用和胞饮作用这两个自然的生物过程完成的。 因此，与破坏、产生孔或电击细胞膜的其它物理转染方法相比，膜的结构保持完整。然后，根据所使用的配方，通过不同的机制将核酸释放到细胞质中。先是由包被在纳米颗粒上的阳离子聚合物引起的质子海绵效应，其促进核内体渗透膨胀、破坏核内体膜和细胞内DNA释放。 第二是核内体被包被在颗粒上的阳离子脂质破坏，这些颗粒通过细胞负性脂质的翻转和电荷中和将核酸释放到细胞中。 第三是病毒使用时常见的病毒机制。

来源：https://www.amyjet.com/products/OZB-MF10096.shtml