样品稀释液：ICT-959样品稀释优化包注意事项：

为了快速确定哪种样品稀释剂配方适合测定，所有三种样品稀释剂都可以在同一IgG或抗原包被的ELISA板上平行运行。

确定在三种不同的样品稀释剂类型中制备的ELISA曲线的初始动态分析物范围。

在血清样本中加入测试尖峰。

读取三条SD相关标准曲线中的每一条上的加标血清样品孔，以查看哪种样品稀释剂提供了添加到血清样品中的加标浓度的佳近似值。

在检测到高水平基质复杂性干扰的情况下，可能还需要加入额外的测定稀释剂试剂，以使校准孔抗体与抗原结合动力学更接近样品孔结合动力学。

ICT样本稀释液：ICT-647一般血清稀释剂引文：

To quickly determine which Sample Diluent formulation is best for the assay, all three Sample Diluents can be run in parallel on the same IgG or antigen-coated ELISA plate.

Determine the initial dynamic analyte range of the ELISA curves that were prepared in each of the three different Sample Diluent types.

Make test spikes into serum samples.

Read the spiked serum sample wells on each of the three SD-associated standard curves to see which Sample Diluent gives the best approximation of the spike concentration that was added to the serum samples.

In cases where a high level of matrix complexity interference is detected, inclusion of an additional Assay Diluent reagent may also be required to bring the calibrator well antibody-to-antigen binding kinetics closer to the sample well binding kinetics.

来源：https://www.amyjet.com/products/ICT-647.shtml