This non-therapeutic biosimilar antibody uses the same variable regions from the therapeutic antibody Pembrolizumab making it ideal for research use. This Pembrolizumab biosimilar reacts with human PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 ａnd CD8 thymocytes as well as activated T ａnd B lymphocytes ａnd myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 ａnd PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, ａnd T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance ａnd prevention of autoimmune disease. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, ａnd breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In experimental models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 ａnd its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being used as cancer treatments. Pembrolizumab binds to PD-1 on activated immune cells to selectively block the interaction of PD-1 with its ligands.

RMP1-14单克隆抗体与小鼠PD-1（程序性死亡-1）反应，也称为CD279。PD-1是由Pdcd50基因编码的55-1 kDa细胞表面受体，属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上短暂表达。PD-1在成功消除抗原后表达下降。此外，Pdcd1 mRNA在B细胞阶段的发育B淋巴细胞中表达。

BioXCell艾美捷体内单克隆抗小鼠 PD-1(CD279)抗体规格：

#  BXC-BE0146

Isotype Human IgG4

Recommended Isotype Control(s) RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Human PD-1

Reported Applications Blocking of PD-1/PD-L signaling

Functional assays

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <1EU/mg (<0.001EU/μg)

Determined by LAL gel clotting assay

Aggregation <5%

Determined by DLS

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from cell culture supernatant

Purification Protein A

RRID AB_2894731

Molecular Weight 150 kDa

Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同种型 大鼠IgG2a， κ

推荐的同种型对照 体内MAb大鼠IgG2a同种型对照，抗三硝基苯酚

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

免疫原 用小鼠PD-1 cDNA转染的叙利亚仓鼠BKH细胞

报告的应用程序 PD-1/PD-L信号传导的体内阻断

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <2EU/mg （<0.002EU/μg）

通过LAL凝胶凝血测定法测定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μM 过滤

生产 从无动物设施中的组织培养上清液中纯化

纯化 蛋白 G

里德 AB_10949053

分子量 150 千达

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

来源：https://www.amyjet.com/products/BXC-CP162-1mg.shtml