这种竞争性ELISA试剂盒用于测定细胞中游离和葡萄糖醛酸化的20-HETE水平提取物和细胞培养基。该试剂盒类似于我们的20-HETE ELISA试剂盒（Cat#20H1），但也包含β-葡萄糖醛酸酶。每个96孔板足以进行多达12个游离样品的一式三份分析和12个葡萄糖醛酸化样品。正常大鼠肾脏（NRK）细胞的花生四烯酸（AA）依赖性20-HETE形成活性100人中20-HETE形成的ELISA测量mL培养基（总体积的1/10）和随后的细胞裂解物培养5 x 104，在含有或不含有100µM AA和1mM NADPH的情况下将细胞培养30分钟。

Detroit R&D 20-HETE/葡萄糖醛酸酶ELISA试剂盒相关研究： 

用于一步纯化的20-HETE免疫亲和柱 20H33

生物靶标 20-HETE ELISA 试剂盒 20H1

Detroit R&D 20-HETE/葡萄糖醛酸酶ELISA试剂盒部分参考文献：

1. Liu et al. Association of a functional cytochrome P450 4F2 haplotype with urinary 20-HETE ａnd hypertension. J. Am. Soc. Nephrol. 19, 714-721, 2008.

2. Meseguer et al. Kidney androgen-regulated protein transgenic mice show hypertension ａnd renal alterations mediated by oxidative stress.Circulation. 119, 1908-1917, 2009.

3. Dolegowska, B., Blogowski, W., Domanski, L.. Is it possible to predict the early post-transplant allograft function using 20-HETE measurements? A preliminary report. Transpl Int. 2009 22:546-553

4. Liu et al. Overexpression of cytochrome P450 4F2 in mice increases 20-hydroxyeicosatetraenoic acid production ａnd arterial blood pressure. Kidney. International. 75, 1288-1296, 2009.

5. Wang et al. Selective inhibitors of CYP2J2 related to terfenadine exhibit activity strongly against human cancers in vitro ａnd in vivo. J.Pharmacol. Exp. Ther. 329, 908-918, 2009.

6. Tunctan et al. A synthetic analogue of 20-HETE, 5,14-HEDGE, reverses endotoxin-Induced hypotension via increased 20-HETE levels associated with decreased iNOS protein expression ａnd vasodilator prostanoid production in rats. Basic Clin. Pharmacol. Toxicol. 106, 378-388, 2010.

Detroit R&D注于心血管疾病、癌症的研究，诊断和治疗域。涉及环境健康科学、高血压、乳腺癌和列腺癌相关研究域。艾美捷科技是Detroit R&D的中国代理商，为科研工作者提供优质的产品与服务。

来源：https://www.amyjet.com/news/Detroit-R-D-new.shtml