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Gibco 31800-022 RPMI1640干粉培养基（含L-谷氨酰胺，不含HEPES，丙酮酸钠，碳酸氢钠）
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详细内容
英文名称：RPMI 1640 Medium, Powder
规格：10 x 1 L
性状：干粉培养基
货号：31800-022
RPMI1640干粉培养基 Gibco试剂 产品描述：
描述
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension ａnd as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes ａnd carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red | • Sodium Bicarbonate |
The complete formulation is available.
Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione ａnd high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle＇s Minimal Essential Medium or Dulbecco＇s Modified Eagle Medium. In addition, the vitamins inositol ａnd choline are present in very high concentrations.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
cGMP Manufacturing ａnd Quality System
Gibco® RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer ａnd is certified to ISO 13485 ａnd ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® RPMI 1640 product made in our Scotland facility (51800-019). This facility is registered with the FDA as a medical device manufacturer ａnd is certified to the ISO 13485 standard.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g / L) ａnd therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, ａnd filtration at the time of preparation (see protocol for details).
RPMI1640干粉培养基 Gibco试剂 产品规格：
规格
| Glutamine: | L-Glutamine |
|---|---|
| Phenol Red Indicator: | Phenol Red |
| Form: | Powder |
| HEPES Buffer: | No HEPES |
| Serum Supplementation: | Standard Serum Supplementation |
| Sodium Pyruvate Additive: | No Sodium Pyruvate |
| Product Size: | 10 x 1 L |
| Shipping Condition: | Room Temperature |
