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GIbco 31985-070 Opti-MEM I 减血清培养基（含L-谷氨酰胺） 31985070
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品牌名称：$brandModel.Title(进口品牌)型号：500ml 原产地：中国大陆 发布时间：2024/4/8 15:04:26更新时间：2024/9/30 11:32:14
产品摘要：产品又名：Gibco 还原血清细胞培养基、Gibco 还原血清细胞培养基英文名称：Gibco Opti-MEM I Reduced Serum Medium培养基类型：哺乳细胞培养基别：For Mammalian Cell Culture品牌：Gibco，Opti-MEM产品目录号：31985070，31985-070谷氨酰胺：含L-谷氨酰胺
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详细内容
Gibco 还原血清细胞培养基产品描述：
Opti MEM®I还原血清培养基是一种改良的低必需培养基（MEM），允许在生长速率或形态没有变化的情况下，将胎牛血清补充量减少至少50%。Opti MEM®I培养基也建议与阳离子脂质转染试剂（如脂质体）一起使用™ 试剂。Opti MEM®I培养基可用于多种悬浮和粘附哺乳动物细胞，包括Sp2、AE-1、CHO、BHK-21、HEK和原代成纤维细胞。我们为一系列细胞培养应用提供各种Opti MEM®I修饰。Gibco 还原血清培养基使用方法
Opti MEM®I还原血清培养基是一种独特的培养基，含有胰岛素、转铁蛋白、次黄嘌呤、胸腺嘧啶核苷和微量元素。这些额外的成分可以减少至少50%的血清补充量。Opti MEM®I培养基使用碳酸氢钠缓冲系统（2.4 g/L），因此需要5–10%的二氧化碳环境来维持生理pH值。cGMP生产与质量体系
Opti MEM®I是在纽约格兰德岛的cGMP兼容工厂生产的。该设施在FDA注册为医疗器械制造商，并通过ISO 13485标准认证。为了供应链的连续性，我们提供了在苏格兰工厂（31985-047）生产的相同Opti MEM®I产品。该设施还作为医疗器械制造商在FDA注册，并通过ISO 13485标准认证。Gibco细胞培养基选择指南
| Transfection Reagent （细胞转染试剂） | ||
|---|---|---|
| 产品描述 | 规格 | 货号 |
| Lipofectamin 2000 （Lipo2000、Lipo 2000） Each unit contains one vial of Lipofectamine 2000 Reagent. | 0.75 ml | 11668-027 11668027 |
| Lipofectamin 3000 （Lipo3000、lipo 3000） Contains one vial of Lipofectamine 3000 Reagent ａnd one vial of P3000TM Enhancer Reagent. | 0.75 ml | L3000-008 L3000008 |
| Dulbecco’s Modified Eagle Media （DMEM培养基） | ||
| 产品描述 | 规格 | 货号 |
| DMEM (1X), liquid (high glucose) Contains no L-glutamine or sodium pyruvate | 500 mL | 11960-044 11960044 |
| DMEM (1X), liquid (high glucose) Contains L-glutamine, but no sodium pyruvate | 500 mL | 11965-092 11965092 |
| DMEM (1X), liquid (high glucose) (DMEM高糖培养基，常用) Contains L-glutamine ａnd sodium pyruvate | 500 mL | 11995-065 11995065 |
| DMEM (1X), liquid (high glucose) Contains 110mg/L sodium pyruvate, but no L-glutamine | 500 mL | 10313-021 10313021 |
| DMEM (1X), liquid (high glucose) Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red | 500 mL | 21063-029 21063029 |
| DMEM (1X), liquid (high glucose) Contains no L-glutamine, sodium pyruvate, or phenol red | 500 mL | 31053-028 31053028 |
| DMEM (1X), liquid (low glucose) Contains L-glutamine ａnd sodium pyruvate. | 500 mL | 11885-084 11885084 |
| DMEM (1X), liquid (high glucose) Contains L-glutamine, ａnd 25 mM HEPES, but no sodium pyruvate | 500 mL | 12430-054 12430054 |
| DMEM/F12 (1X), liquid 1:1 Contains L-glutamine, 15 mM HEPES butter, but no phenol red | 500 mL | 11039-021 11039021 |
| DMEM/F12 (1X), liquid 1:1 Contains L-glutamine, but no HEPES buffer | 500 mL | 11320-033 11320033 |
| DMEM/F12 (1X), liquid 1:1 Contains L-glutamine ａnd 15 mM HEPES buffer | 500 mL | 11330-032 11330032 |
| RPMI Media （RPMI1640培养基） | ||
| 产品描述 | 规格 | 货号 |
| RPMI 1640 (1X), liquid（常用） Contains L-glutamine | 500 mL | 11875-093 11875093 |
| RPMI 1640 (1X), liquid Contains L-glutamine, but no phenol red | 500 mL | 11835-030 11835030 |
| RPMI 1640 (1X), liquid Contains no L-glutamine | 500 mL | 21870-076 21870076 |
| RPMI 1640 (1X), liquid Contains L-glutamine ａnd 25 mM HEPES buffer | 500 mL | 22400-089 22400089 |
| Minimum Essential Media （MEM培养基） | ||
| 产品描述 | 规格 | 货号 |
| MEM Earle’s (1X), liquid Contains Earle’s salts ａnd L-glutamine | 500 mL | 11095-080 11095080 |
| MEM Earle’s (1X), liquid Contains Earle’s salts ａnd non-essential amino acids, but no L-glutamine | 500 mL | 10370-021 10370021 |
| MEM Earle’s (1X), liquid Contains Earle’s salts, but no L-glutamine | 500 mL | 11090-081 11090081 |
| MEM α (1X), liquid Contains L-glutamine, ribonucleosides, ａnd deoxyribonuclosides | 500 mL | 12571-063 12571063 |
| MEM α Medium (1X), liquid Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides | 500 mL | 12561-056 12561056 |
| MEM α Medium (1X), liquid Contains L-glutamine, ribonucleosides ａnd deoxyribonucleosides, but no phenol red | 500 mL | 41061-029 41061029 |
| GlutaMAX™ Media （GlutaMAX系列培养基） | ||
| 产品描述 | 规格 | 货号 |
| DMEM (1X), liquid (high glucose) Contains 110 mg/L sodium pyruvate | 500 mL | 10569-010 10569010 |
| DMEM (1X), liquid (high glucose) Contains 25 mM HEPES buffer, but no sodium pyruvate | 500 mL | 10564-011 10564011 |
| DMEM (1X), liquid (high glucose) Contains no sodium pyruvate | 500 mL | 10566-016 10566016 |
| DMEM (1X), liquid (low glucose) Contains 110 mg/L sodium pyruvate | 500 mL | 10567-014 10567014 |
| DMEM/F12 (1X), liquid 1:1 Contains no HEPES buffer | 500 mL | 10565-018 10565018 |
| F12 Nutrient Mixture (1X), liquid | 500 mL | 31765-035 31765035 |
| IMDM (1X), liquid Contains 25 mM HEPES buffer ａnd 3,024 mg/L sodium bicarbonate | 500 mL | 31980-030 31980-030 |
| MEM α (1X), liquid （常用） Contains no ribonucleosides or deoxyribonucleosides | 500 mL | 32561-037 32561037 |
| MEM α (1X), liquid Contains ribonucleosides ａnd deoxyribonucleosides | 500 mL | 32571-036 32571036 |
| MEM Earle’s (1X), liquid Contains Earle’s salts | 500 mL | 41090-036 41090036 |
| MEM Earle’s (1X), liquid Contains Earle’s salts ａnd 25 mM HEPES buffer | 500 mL | 42360-032 42360032 |
| OptiMEM® I Reduced Serum Medium (1X), liquid | 500 mL | 51985-034 51985034 |
| RPMI 1640 (1X), liquid | 500 mL | 61870-036 61870036 |
| RPMI 1640 (1x), liquid Contains 25 mM HEPES buffer | 500 mL | 72400-047 72400047 |
| 其他常用基础培养基 | ||
| 产品描述 | 规格 | 货号 |
| F12 Nutrient Mixture (1X), liquid Contains L-glutamine | 500 mL | 11765-054 11765054 |
| F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid Contains L-glutamine | 500 mL | 21127-022 21127022 |
| IMDM (1X), liquid Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol | 12440-053 12440053 | |
| Liebovitz’s L-15 Medium (1X), liquid (L15 Medium) Contains L-glutamine | 11415-064 11415064 | |
| McCoy’s 5A Medium (modified) (1X), liquid Contains L-glutamine | 16600-082 16600082 | |
| Medium 199 (1X), liquid Contains Earle’s salts, L-glutamine, ａnd 2,200 mg/L sodium bicarbonate | 11150-059 11150059 | |
| Reduced Serum Media 细胞减血清培养基 | ||
| 产品描述 | 规格 | 货号 |
| Advanced D-MEM (1X), liquid | 500 mL | 12491-015 12491015 |
| Advanced D-MEM/F12 (1X), liquid | 500 mL | 12634-010 12634010 |
| Advanced MEM (1X), liquid | 500 mL | 12492-013 12492013 |
| Advanced RPMI 1640 (1X), liquid | 500 mL | 12633-012 12633012 |
| Opti-MEM®-I Reduced Serum Medium (1X), liquid | 500 mL | 31985-070 31985070 |
| Balanced Salt Solutions 细胞培养缓冲液 | ||
| 产品描述 | 规格 | 货号 |
| DPBS (1X), liquid Contains calcium ａnd magnesium | 500 mL | 14040-133 14040133 |
| DPBS (1X), liquid Contains no calcium or magnesium | 500 mL | 14190-144 14190144 |
| DPBS (10X), liquid Contains no calcium or magnesium | 500 mL | 14200-075 14200075 |
| HBSS (1X), liquid Contains calcium ａnd magnesium, but no phenol red | 500 mL | 14025-092 14025092 |
| HBSS (1X), liquid Contains no calcium or magnesium | 500 mL | 14170-112 14170112 |
| HBSS (1X), liquid Contains no calcium chloride, magnesium chloride, magnesium sulfate, or phenol red | 500 mL | 14175-095 14175-095 |
| PBS 7.4 (1X), liquid | 500 mL | 10010-023 10010023 |
| PBS 7.4 (10X), liquid | 500 mL | 70011-044 70011044 |
| PBS 7.2 (1X), liquid | 500 mL | 20012-027 20012027 |
| PBS 7.2 (10X), liquid | 500 mL | 70013-032 70013032 |
| Insect Media 昆虫培养基 | ||
| 产品描述 | 规格 | 货号 |
| Grace’s Insect Medium, Supplemented (1X), liquid Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, ａnd L-glutamine, but no insect hemolymph | 500 mL | 11605-094 11605094 |
| Schneider’s Drosophila Medium (1X), liquid Contains L-glutamine | 500 mL | 11720-034 11720034 |
| SF900-II SFM (1X), liquid Contains L-Glutamine, Insect cell culture tested | 1000 mL | 10902-088 10902088 |
| Reagents 细胞培养试剂 | ||
| 产品描述 | 规格 | 货号 |
| TrypLE™ Express Dissociation Enzyme with Phenol Red Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA | 100 mL | 12605-010 12605010 |
| TrypLE™ Express Dissociation Enzyme without Phenol Red Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA | 100 mL | 12604-013 12604013 |
| Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid（胰蛋白酶，常用） Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, ａnd MgSO4•7H2O. Contains phenol red | 100 mL | 25200-056 25200056 |
| Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, ａnd MgSO4•7H2O. Contains phenol red | 100 mL | 25300-054 25300054 |
| HEPES Buffer Solution (1M) Contains 238.3 g/L prepared in distilled water | 100 mL | 15630-080 15630080 |
| L-glutamine-200 mM (100X), liquid | 100 mL | 25030-081 25030081 |
| GlutaMAX™-I (100X), liquid | 100 mL | 35050-061 35050061 |
| Antibiotic-Antimycotic (100X), liquid 10,000 units penicillin (base), 10,000 units streptomycin (base), ａnd 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, ａnd amphotericin B as Fungizone™ Antimycotic in 0.85% saline | 100 mL | 15240-062 15240062 |
| Penicillin-Streptomycin, liquid （青链霉素双抗） 10,000 Units penicillin (base) ａnd 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) ａnd streptomycin sulfate in 0.85% saline | 100 mL | 15140-122 15140122 |
| MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid | 100 mL | 11140-050 11140050 |
| Distilled Water（无菌水） Membrane filtered ａnd endotoxin screened | 500 mL | 15230-162 15230162 |
