产品货号
XY2696
产品名称CRYAB抗体
中文名称α晶状体球蛋白B/αB-crystallin抗体
蛋白名Alpha-crystallin B chain
别称MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16
CRYAB抗体
来源小鼠
特异性抗人
分子量-
克隆类型单克隆抗体：IgG2a；克隆号：1D11C6E6
应用IH(P)免疫组化(石蜡)；ELISA
成分Ascitic fluid containing 0.03% sodium azide.
免疫原Purified recombinant fragment of CRYAB (aa1-175) expressed in E. Coli.
保存条件连续使用时4°C存储，保质期六个月；长期存储时建议分装为10ul以上小包装-20°C存储，并避免反复冻融，保质期一年。
背景资料Crystallin, alpha B. Crystallins are separated into two classes: taxon-specific, or enzyme, ａnd ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens ａnd maintains the transparency ａnd refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made ａnd then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, ａnd gamma families; beta ａnd gamma crystallins are also considered as a superfamily. Alpha ａnd beta families are further divided into acidic ａnd basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, ａnd N- ａnd C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A ａnd alpha-B, for acidic ａnd basic, respectively. Alpha crystallins can be induced by heat shock ａnd are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins ａnd release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A ａnd alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity ａnd participation in the intracellular architecture. Alpha-A ａnd alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens ａnd alpha-B is expressed widely in many tissues ａnd organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. 

CRYAB抗体参考效价：

Flow Cytometry:1:40-80

Western blotting:1:100-400

Immunocytochemistry in fixed cells:1:50-200

enzyme-linked immuno sorbent assay:1:2000

Immunohistochemistry in paraffin section:1:50-200

CRYAB抗体的保存建议：

抗体保存得当与否直接决定了抗体的活性和使用效果。如果抗体保存得当，大部分抗体活性都可以维持数月甚至数年。请按照说明书或者抗体标签上推荐的保存条件正确保存抗体！无论是保存还是运输，绝对避免反复冻融抗体！

1.拿到抗体后请务必在1000-3000转离心1-2分钟后再打开管盖进行分装和保存！（由于运输过程中反复颠簸，管内微量抗体容易聚集在管盖处，一旦直接打开容易流失抗体。请放心抗体出厂时保证已装入足量。）

2.对于大部分抗体，比较合适的保存方式是分装后保存在-20℃冰箱。

◇分装可以程度降低反复冻融对抗体活性的损害，同时也降低了多次从同一管中吸取抗体造成污染的可能性。

◇分装的量以一次实验用完为好，但建议*少不要少于10μL每份。分装体积越小，抗体浓度可能会受到蒸发以及管壁吸附的影响，同时分装次数越多移液吸头吸附的抗体也越多，可能会误认为抗体没有足量装入。（用无菌的进口0.2ML离心管来分装抗体，国产的小管一般没有经过很好的硅化处理，容易吸附抗体蛋白，影响抗体活性。）

◇分装时请将无菌的微量移液器吸头先插入管底部反复吹打几次后再吸出抗体，以便抗体有效成分与抗体保护剂充分混匀。

◇复融后的分装抗体如果一次用不完，请将剩余母液保存在4℃冰箱，避免再冻起来！

◇绝对避免将抗体保存在自动除霜冰箱的冷藏室中。CRYAB抗体尽量将抗体保存在手动除霜冰箱里面，而不是在冰箱门上。

3.大部分抗体收到后4℃短暂保存1-2周对抗体活性是基本没有影响的。如果抗体很快（1-2周内）就能使用完，推荐在4℃保存，避免反复冻融对抗体活性的损害。如果要长期保存则分装后-20℃保存。

4.即用型抗体请务必保存在4℃冰箱，一定不能冷冻保存。

5.特殊抗体的保存：

◇酶联抗体：一般保存在4℃，尽量避免冷冻起来。否则可能会导致酶活力的下降或者丧失。

◇偶联抗体：所有偶联抗体需要4℃避光保存。尤其是荧光标记抗体，对光极为敏感，因此所有实验阶段也是要避光操作的。