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laminin γ2抗体
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抗体
蛋白/多肽/抗原
原代细胞
细胞系
分子生物学
生化试剂
标准品/对照品
详细内容
产品名称 laminin γ2抗体
蛋白名Laminin subunit gamma-2
别称BM600-100kDa, kalinin-105kDa, nicein-100kDa
laminin γ2抗体
免疫原多肽
来源兔
特异性抗人,大鼠,小鼠
克隆类型多克隆
应用IH(P)免疫组化(石蜡);IH(F)免疫组化(冰冻);IC细胞免疫化学;WB免疫印迹
成分200μg/ml亲和纯化抗体,0.01M PBS,30%甘油。
保存条件连续使用时4°C存储,保质期六个月;长期存储时建议分装为10ul以上小包装-20°C存储,并避免反复冻融,保质期一年。
背景资料Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.
参考效价:
抗体保存得当与否直接决定了抗体的活性和使用效果。如果抗体保存得当,大部分抗体活性都可以维持数月甚至数年。请按照说明书或者抗体标签上推荐的保存条件正确保存抗体!无论是保存还是运输,绝对避免反复冻融抗体!
1.拿到抗体后请务必在1000-3000转离心1-2分钟后再打开管盖进行分装和保存!(由于运输过程中反复颠簸,管内微量抗体容易聚集在管盖处,一旦直接打开容易流失抗体。请放心抗体出厂时保证已装入足量。)
2.对于大部分抗体,比较合适的保存方式是分装后保存在-20℃冰箱。
◇分装可以程度降低反复冻融对抗体活性的损害,laminin γ2抗体同时也降低了多次从同一管中吸取抗体造成污染的可能性。
◇分装的量以一次实验用完为好,但建议*少不要少于10μL每份。分装体积越小,抗体浓度可能会受到蒸发以及管壁吸附的影响,同时分装次数越多移液吸头吸附的抗体也越多,可能会误认为抗体没有足量装入。(用无菌的进口0.2ML离心管来分装抗体,国产的小管一般没有经过很好的硅化处理,容易吸附抗体蛋白,影响抗体活性。)
◇分装时请将无菌的微量移液器吸头先插入管底部反复吹打几次后再吸出抗体,laminin γ2抗体以便抗体有效成分与抗体保护剂充分混匀。
◇复融后的分装抗体如果一次用不完,请将剩余母液保存在4℃冰箱,避免再冻起来!
◇绝对避免将抗体保存在自动除霜冰箱的冷藏室中。尽量将抗体保存在手动除霜冰箱里面,而不是在冰箱门上。
3.大部分抗体收到后4℃短暂保存1-2周对抗体活性是基本没有影响的。如果抗体很快(1-2周内)就能使用完,推荐在4℃保存,避免反复冻融对抗体活性的损害。如果要长期保存则分装后-20℃保存。
4.即用型抗体请务必保存在4℃冰箱,一定不能冷冻保存。
5.特殊抗体的保存:
◇酶联抗体:一般保存在4℃,尽量避免冷冻起来。否则可能会导致酶活力的下降或者丧失。
◇偶联抗体:所有偶联抗体需要4℃避光保存。尤其是荧光标记抗体,对光极为敏感,因此所有实验阶段也是要避光操作的。
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