FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

 

SEQUENCES

The target protein is fused with two N-terminal Tags, His-tag ａnd T7-tag, its sequence is listed below.
SGEDEQQEQ TIAEDLVVTK YKMGGDIANR VLRSLVEASS SGVSVLSLCE KGDAMIMEET GKIFKKEKEM KKGIAFPTSI SVNNCVCHFS PLKSDQDYIL KEGDLVKIDL GVHVDGFIAN VAHTFVVDVA QGTQVTGRKA DVIKAAHLCA EAALRLVKPG NQNTQVTEAW NKVAHSFNCT PIEGMLSHQL KQHVIDGEKT IIQNPTDQQK KDHEKAEFEV HEVYAVDVLV SSGEGKAKDA  增殖关联蛋白2G4(PA2G4)重组蛋白GQRTTIYKRD PSKQYGLKMK TSRAFFSEVE RRFDAMPFTL RAFEDEKKAR MGVVECAKHE LLQPFNVLYE KEGEFVAQFK FTVLLMPNGP MRITSGPFEP DLYKSEMEVQ DAELKALLQS SASRKTQKKK KKKASKTAEN ATSGETLEEN EAGD

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot ａnd store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, ａnd no obvious degradation ａnd precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa ａnd 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location ａnd size approximation of proteins of interest quick ａnd easy.
 增殖关联蛋白2G4(PA2G4)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.XY135Ga01血小板生成素(TPO)重组蛋白Recombinant Thrombopoietin (TPO)Chicken (Gallus)