FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

 

SEQUENCES

 特异性蛋白1(Sp1)重组蛋白The target protein is fused with two N-terminal Tags, His-tag ａnd GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- L NGNITLLPVN SVSAATLTPS SQAVTISSSG SQESGSQPVT SGTTISSASL VSSQASSSSF FTNANSYSTT TTTSNMGIMN FTTSGSSGTN SQGQTPQRVS GLQGSDALNI QQNQTSGGSL QAGQQKEGEQ NQQTQQQQIL IQPQLVQGGQ ALQALQAAPL SGQTFTTQAI SQETLQNLQL QAVPNSGPII IRTPTVGPNG QVSWQTLQLQ NLQVQNPQAQ TITLAPMQGV SLGQTSSSNT TLTPIASAAS IPAGTVTVNA AQLSSMPGLQ TINLSALGTS GIQVHPIQGL PLAIANAPGD HGAQLGLHGA GGDGIHDDTA GGEEGENSPD A

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot ａnd store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, ａnd no obvious degradation ａnd precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa ａnd 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location ａnd size approximation of proteins of interest quick ａnd easy.
 特异性蛋白1(Sp1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.XY223Hu01白介素1受体拮抗剂(IL1RA)重组蛋白Recombinant Interleukin 1 Receptor Antagonist (IL1RA)Homo sapiens (Human)