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粒细胞分化相关标志物MYADM抗体说明书
点击次数:55发布时间:2016/9/13 9:47:56
更新日期:2024/8/5 18:43:00
所 在 地:中国大陆
产品型号:
品牌名称:上海雅吉
相关标签:粒细胞分化相关标志物MYADM抗体说明书
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详细内容
为满足客户对抗目标靶标物单克隆抗体的迫切需求,单抗制备团队经过多年的系统深入研究,发现了外源细胞因子对杂交瘤形成与生长调节规律,提出了一种全新技术方案,通过探明人类干细胞因子bFGF、HFCS对目标多肽以及靶标蛋白阳性杂交瘤形成与生长调节规律,创建了杂交瘤细胞株半固体干细胞培养基-梯度筛选法(简称"两步筛选法"),突破了常规HAT、HT培养筛选法阳性率低、随机性大的局限。单克隆抗体制备服务*快可在100天内完成单抗制备过程,并且保证为客户提供2个阳性克隆以及高亲和力的抗体产品。
公司可根据客户的不同需要提供经济、快速而可靠的单克隆抗体制备的完整解决方案,本项技术服务包括:
- Balb/c纯系小鼠免疫;
- 脾细胞与SP2/0骨髓瘤细胞的融合;
- 抗体产生细胞的ELISA筛选;
- 抗体产生细胞的亚克隆与扩增;
- 指定细胞克隆的体外培养或腹水瘤接种法生产单克隆抗体
产品特点
英文名称:Anti-Salmonella typhimurium O/Cy3
英文名称:Anti-Salmonella typhimurium O/Cy3
中文名称:粒细胞分化相关标志物MYADM抗体说明书
别 名:Silent protein UshA(0); USHA_SALTY.
说 明 书:100ul
研究领域:细菌及病毒
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Mouse, SalnellatyphimuriumO
产品应用:ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量:58kDa
性 状:Lyophilized or Liquid
浓 度:1mg/ml
免 疫 原:whole cell protein of Salmonella typhimurium O
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram-negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) and the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core and the endotoxic inner "Lipid A". Endotoxins evoke fever and can activate complement, kinin and clotting factors. Until recently the most common cause of food poisoning by Salmonella species was due to S. Typhimurium. As its name suggests, it causes a typhoid-like disease in mice. In humans S. Typhimurium does not cause as severe disease as S. Typhi, and is not normally fatal. The disease is characterized by diarrhea, abdominal cramps, vomiting and nausea, and generally lasts up to 7 days.
Subcellular Location:
Periplasm (By similarity).
Similarity:
Belongs to the 5'-nucleotidase family.
Database links:
UniProtKB/Swiss-Prot: P06196.2
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
公称型号
YS-8660RSalmonella typhimurium O伤寒沙门氏菌体蛋白O抗原抗体一抗0.1ml
YS-8670RSalmonella enteritidis H伤寒沙门氏菌鞭毛抗原H抗体一抗0.1ml
YS-8689RMEPE细胞外基质磷酸化抗体一抗0.1ml
YS-0879RFLAG Tag (CT)FLAG Tag标签(C端)抗体一抗0.1ml
YS-9769RC10orf6210号染色体开放阅读框62抗体一抗
YS-8672RHDL高密度脂蛋白抗体一抗0.1ml
YS-17822RMRPS25线粒体核糖体蛋白S25抗体一抗
YS-17823RMRPS26线粒体核糖体蛋白S26抗体一抗0.1ml
YS-17824RMRPS27线粒体核糖体蛋白S27抗体一抗
YS-17825RMRPS28线粒体核糖体蛋白S28抗体一抗
YS-17826RMRPS30线粒体核糖体蛋白S30抗体一抗
YS-17827RMRPS31线粒体核糖体蛋白S31抗体一抗0.1ml
YS-17828RMRPS34线粒体核糖体蛋白S34抗体一抗
YS-17829RMRPS35线粒体核糖体蛋白S35抗体一抗
YS-17830RMRPS36线粒体核糖体蛋白S36抗体一抗
YS-17831RMRPS5线粒体核糖体蛋白S5抗体一抗
YS-17832RMRPS6线粒体核糖体蛋白S6抗体一抗
YS-17833RMRPS7线粒体核糖体蛋白S7抗体一抗
YS-17834RMRPS9线粒体核糖体蛋白S9抗体一抗
YS-17835RMRRF核糖体再循环因子MRRF抗体一抗0.1ml
YS-7467RPIK3R5磷脂酰肌醇激酶催化亚单位p101抗体一抗0.1ml
YS-17836RMRS2MRS2蛋白抗体一抗
YS-17837RMRVI1MRVI1蛋白抗体一抗
YS-17838RMS4A12MS4A12蛋白抗体一抗
YS-17840RCD20L1/MS4A4ACD20样蛋白1抗体一抗
YS-17841RMS4A4EMS4A4E蛋白抗体一抗
YS-17842RCD20L2/MS4A5CD20样蛋白2抗体一抗
YS-17843RMS4A6EMS4A6E蛋白抗体一抗
YS-17844RCD20L4/MS4A7CD20样蛋白4抗体一抗
YS-17855Rphospho-MSK2 (Thr568)磷酸化有丝分裂原和应激活化型蛋白激酶2抗体一抗0.1ml
YS-17865RMT-ATP6ATP6蛋白抗体一抗0.1ml
YS-17898RMTX2MTX2蛋白抗体一抗
YS-17911ROR52W1嗅觉受体52W1抗体一抗
YS-17912ROR56A1嗅觉受体56A1抗体一抗
YS-17926ROR5AU1嗅觉受体5AU1抗体一抗
YS-17845RCD20L5/MS4A8BCD20样蛋白5抗体一抗
YS-17846RMSANTD2/C11orf6111号染色体开放阅读框61抗体一抗
粒细胞分化相关标志物MYADM抗体说明书溶解方法
方法1:我们的抗体(冻干粉)已经包含了0.01M PBS、1% BSA和0.1% 防腐剂(叠氮钠或庆大霉素),您只需要加入灭菌的双蒸水就行了。抗体溶解后,置于-20℃保存,分装后使用,避免反复冻融,可保证至少1年有效;
方法2:也可以只加入一半体积的双蒸水,再用一半体积的甘油补足,置于-20℃保存,这样抗体不会冻,有利于抗体的稳定。
后续试验时,再使用对应的缓冲液稀释成工作液,即用即配。
我们的抗体(冻干粉),在常温放置下,至少一个月有效;若在-20℃下保存(推荐),至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
抗体修复方式
修复液:0.01M 枸橼酸(pH6.0)或者0.05M EDTA(pH8.0)
粒细胞分化相关标志物MYADM抗体说明书抗体修复方法:
方法1:沸水浴修复,将盛有修复液和玻片的烧杯置于沸水浴环境,保持外部沸腾状态15min,自然冷却至室温;
方法2:微波修复,将盛有修复液和玻片的烧杯置于微波炉中,高火5min,停火3min,中火5min,自然冷却至室温。