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紧密连接蛋白14抗体说明书
点击次数：36发布时间：2016/9/14 11:04:17
更新日期：2024/8/5 18:43:00
所 在 地：中国大陆
产品型号：
品牌名称：上海雅吉
相关标签：紧密连接蛋白14抗体说明书
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详细内容
为满足客户对抗目标靶标物单克隆抗体的迫切需求,单抗制备团队经过多年的系统深入研究，发现了外源细胞因子对杂交瘤形成与生长调节规律，提出了一种全新技术方案，通过探明人类干细胞因子bFGF、HFCS对目标多肽以及靶标蛋白阳性杂交瘤形成与生长调节规律，创建了杂交瘤细胞株半固体干细胞培养基-梯度筛选法（简称"两步筛选法"），突破了常规HAT、HT培养筛选法阳性率低、随机性大的局限。单克隆抗体制备服务*快可在100天内完成单抗制备过程，并且保证为客户提供2个阳性克隆以及高亲和力的抗体产品。
公司可根据客户的不同需要提供经济、快速而可靠的单克隆抗体制备的完整解决方案，本项技术服务包括：
- Balb/c纯系小鼠免疫；
- 脾细胞与SP2/0骨髓瘤细胞的融合；
- 抗体产生细胞的ELISA筛选；
- 抗体产生细胞的亚克隆与扩增；
- 指定细胞克隆的体外培养或腹水瘤接种法生产单克隆抗体
产品特点
英文名称：Anti-Salmonella typhimurium O/Cy3
英文名称：Anti-Salmonella typhimurium O/Cy3
中文名称：紧密连接蛋白14抗体说明书
别 名：Silent protein UshA(0); USHA_SALTY.
说 明 书：100ul
研究领域：细菌及病毒
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Mouse, SalnellatyphimuriumO
产品应用：ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量：58kDa
性 状：Lyophilized or Liquid
浓 度：1mg/ml
免 疫 原：whole cell protein of Salmonella typhimurium O
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Histones play a critical role in transcriptional regulation, cell cycle progression, ａnd developmental events. Histone acetylation/deacetylation alters chromosome structure ａnd affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity ａnd represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C ａnd MEF2D. It seems to interact in a multiprotein complex with RbAp48 ａnd HDAC3. [provided by RefSeq, Jul 2008].
Function:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 ａnd H4). Histone deacetylation gives a tag for epigenetic repression ａnd plays an important role in transcriptional regulation, cell cycle progression ａnd developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C ａnd MEF2D.
Subunit:
Interacts with HDAC7. Homodimer. Homodimerization via its N-terminal domain. Interacts with MEF2C, AHRR, ａnd NR2C1. Interacts with a 14-3-3 chaperone protein in a phosphorylation dependent manner. Interacts with BTBD14B. Interacts with KDM5B. Interacts with MYOCD. Interacts with MORC2. Interacts with ANKRA2.
Subcellular Location:
Nucleus. Cytoplasm. Shuttles between the nucleus ａnd the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein ａnd is due to its phosphorylation at Ser-246, Ser-467 ａnd Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Phosphorylated by CaMK4 at Ser-246, Ser-467 ａnd Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350 impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, ａnd prevented by phosphorylation by CaMK4.
DISEASE:
Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, ａnd stocky build. Soft tissue ossification is absent, ａnd there are no abnormalities in parathyroid hormone or calcium metabolism.
Similarity:
Belongs to the histone deacetylase family. HD type 2 subfamily.
Gene ID:
9759
Database links:
Entrez Gene: 9759 Human
Entrez Gene: 208727 Mouse
Entrez Gene: 363287 Rat
Omim: 605314 Human
SwissProt: P56524 Human
SwissProt: Q6NZM9 Mouse
SwissProt: Q99P99 Rat
Unigene: 20516 Human
Unigene: 318567 Mouse
Unigene: 23483 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
公称型号
YS-3212RPhospho-HER2 (Tyr1221 + Tyr1222)磷酸化HER2受体抗体
YS-3127Rphospho-HDAC8 (Ser39)磷酸化组蛋白去乙酰化酶8抗体
YS-11071RANTXR2炭疽毒素受体2抗体
YS-3214RPhospho-HER2(Tyr1248)磷酸化HER2受体抗体
YS-2811RHDAC6组蛋白去乙酰化酶6抗体
YS-3215Rphospho-HDAC6 (Ser22)磷酸化组蛋白去乙酰化酶6抗体
YS-2890RHDAC7组蛋白去乙酰化酶7抗体
YS-3216Rphospho-HDAC7 (Ser358)磷酸化组蛋白去乙酰化酶7抗体
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紧密连接蛋白14抗体说明书溶解方法
方法1：我们的抗体（冻干粉）已经包含了0.01M PBS、1% BSA和0.1% 防腐剂（叠氮钠或庆大霉素），您只需要加入灭菌的双蒸水就行了。抗体溶解后，置于-20℃保存，分装后使用，避免反复冻融，可保证至少1年有效；
方法2：也可以只加入一半体积的双蒸水，再用一半体积的甘油补足，置于-20℃保存，这样抗体不会冻，有利于抗体的稳定。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA ａnd 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized ａnd reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
抗体修复方式
修复液：0.01M 枸橼酸（pH6.0）或者0.05M EDTA（pH8.0）
紧密连接蛋白14抗体说明书抗体修复方法：
方法1：沸水浴修复，将盛有修复液和玻片的烧杯置于沸水浴环境，保持外部沸腾状态15min，自然冷却至室温；
方法2：微波修复，将盛有修复液和玻片的烧杯置于微波炉中，高火5min，停火3min，中火5min，自然冷却至室温。
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