公司介绍为满足客户对抗目标靶标物单克隆抗体的迫切需求,单抗制备团队经过多年的系统深入研究,发现了外源细胞因子对杂交瘤形成与生长调节规律,提出了一种全新技术方案,通过探明人类干细胞因子bFGF、HFCS对目标多肽以及靶标蛋白阳性杂交瘤形成与生长调节规律,创建了杂交瘤细胞株半固体干细胞培养基-梯度筛选法(简称"两步筛选法"),突破了常规HAT、HT培养筛选法阳性率低、随机性大的局限。单克隆抗体制备服务*快可在100天内完成单抗制备过程,并且保证为客户提供2个阳性克隆以及高亲和力的抗体产品。
公司可根据客户的不同需要提供经济、快速而可靠的单克隆抗体制备的完整解决方案,本项技术服务包括:
- Balb/c纯系小鼠免疫;
- 脾细胞与SP2/0骨髓瘤细胞的融合;
- 抗体产生细胞的ELISA筛选;
- 抗体产生细胞的亚克隆与扩增;
- 指定细胞克隆的体外培养或腹水瘤接种法生产单克隆抗体
产品特点英文名称:Anti-Salmonella typhimurium O/Cy3
中文名称:B细胞特异性转录因子抗体说明书
别 名:Silent protein UshA(0); USHA_SALTY.
说 明 书:100ul
研究领域:细菌及病毒
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Mouse, SalnellatyphimuriumO
产品应用:ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量:58kDa
性 状:Lyophilized or Liquid
浓 度:1mg/ml
免 疫 原:whole cell protein of Salmonella typhimurium O
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品相册
| CD163 (K-18) 抗体: sc-18796 | | | | - goat polyclonal IgG, 200 µg/ml
- epitope mapping within an internal region of CD163 of mouse origin
- recommended for detection of CD163 of mouse, rat and, to a lesser extent, human origin by WB, IF and ELISA
- blocking peptide, sc-18796 P
| | | 引 | 种属 | 基因名称 | 基因ID | 染色质位置 | 亚型(mRNA)即位号码 | 蛋白质即位号码 | OMIM™ 号码 | 人类 | CD163 | 9332 | 12p13.31 | NM_004244, NM_203416 | Q86VB7 | 605545 | 小鼠 | Cd163 | 93671 | 6 F2 | | Q2VLH6 | N/A | | 订购信息 产品名称 | 产品编号 | 规格 | 价格 | CD163 (K-18) | sc-18796 | 200 µg/ml | RMB2,099 | | | | CD163 (K-18) P | sc-18796 P (peptide) | 100 µg/0.5 ml | RMB470 | | | | siRNA 基因沉默子 产品名称 | 产品编号 | 规格 | 价格 | CD163 siRNA (m) | sc-42835 | 10 µM | RMB1,986 | | | | CD163 siRNA (h) | sc-42834 | 10 µM | RMB1,986 | | | | CD163 (m)-PR | sc-42835-PR | 10 µM | RMB178 | | | | CD163 (h)-PR | sc-42834-PR | 10 µM | RMB178 | | | | shRNA 质粒 (点击产品名称查看详细信息 ) 产品名称 | 产品编号 | 规格 | 价格 | CD163 shRNA Plasmid (m) | sc-42835-SH | 20 µg | RMB4,012 | | | | CD163 shRNA Plasmid (h) | sc-42834-SH | 20 µg | RMB4,012 | | | | shRNA 慢病毒颗粒 (点击产品名称查看详细信息 ) 产品名称 | 产品编号 | 规格 | 价格 | CD163 shRNA (m) Lentiviral Particles | sc-42835-V | 200 µl | RMB4,822 | | | | CD163 shRNA (h) Lentiviral Particles | sc-42834-V | 200 µl | RMB4,822 | | | | CD163 Background Information CD163, also designated M130, is a macrophage-associated antigen that is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. It is highly expressed on macrogphages and to a lesser extent on monocytes. The acute phase-regulated and signal-inducing macrophage protein, CD163, is a receptor that scavenges hemoglobin by mediating endocytosis of haptoglobin-hemoglobin complexes. CD163 binds only haptoglobin and hemoglobin in complex, which indicates the exposure of a receptor-binding neoepitope. The receptor-ligand interaction is calcium-dependent and of high affinity. The existence of several CD163 isoforms, which differ in the structure of their cytoplasmic domains and putative phosphorylation sites, suggests that these isoforms also differ in their signaling mechanism. The gene which encodes CD163 maps to human chromosome 12p13.31. |
B细胞特异性转录因子抗体说明书介绍:ackground: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008].
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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B细胞特异性转录因子抗体说明书溶解方法
方法1:我们的抗体(冻干粉)已经包含了0.01M PBS、1% BSA和0.1% 防腐剂(叠氮钠或庆大霉素),您只需要加入灭菌的双蒸水就行了。抗体溶解后,置于-20℃保存,分装后使用,避免反复冻融,可保证至少1年有效;
方法2:也可以只加入一半体积的双蒸水,再用一半体积的甘油补足,置于-20℃保存,这样抗体不会冻,有利于抗体的稳定。
后续试验时,再使用对应的缓冲液稀释成工作液,即用即配。
我们的抗体(冻干粉),在常温放置下,至少一个月有效;若在-20℃下保存(推荐),至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
抗体修复方式
修复液:0.01M 枸橼酸(pH6.0)或者0.05M EDTA(pH8.0)
B细胞特异性转录因子抗体说明书抗体修复方法:
方法1:沸水浴修复,将盛有修复液和玻片的烧杯置于沸水浴环境,保持外部沸腾状态15min,自然冷却至室温;
方法2:微波修复,将盛有修复液和玻片的烧杯置于微波炉中,高火5min,停火3min,中火5min,自然冷却至室温。
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