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产品名称: 大秃马勃使用说明书
英文名称:Calvatia gigantea
型号:GOY-JZ0630
培养温度:35-37℃
培养保藏法:培养后于4—6℃冰箱内保存。
大秃马勃使用说明书温保存法:
1.简单保存法,将琼脂斜面孢子培养物、菌丝悬浮液以及由麸皮、大米、小米等谷物原料制成的孢子培养物置于4℃冰箱保存,保存时间不超过1~2个月,若将谷物原料制备的孢子瓶抽真空并在棉塞上浸蜡,以隔绝外界空气和水汽,保持时间可达3~4个月。
保藏方法
1. 传代培养保藏法
又有斜面培养、穿刺培养、疱肉培养基培养等(后者作保藏厌氧细菌用),培养后于4-6℃冰箱内保存。
2. 液体石蜡覆盖保藏法
是传代培养的变相方法,能够适当延长保藏时间,它是在斜面培养物和穿刺培养物上面覆盖灭菌的液体石蜡,一方面可防止因培养基水分蒸发而引起菌种死亡,另一方面可阻止氧气进入,以减弱代谢作用。
3. 载体保藏法
是将微生物吸附在适当的载体,如土壤、沙子、硅胶、滤纸上,而后进行干燥的保藏法,例如沙土保藏法和滤纸保藏法应用相当广泛。
4. 寄主保藏法
用于目前尚不能在人工培养基上生长的微生物,如病毒、立克次氏体、螺旋体等,它们必须在生活的动物、昆虫、鸡胚内感染并传代,此法相当于一般微生物的传代培养保藏法。病毒等微生物亦可用其他方法如液氮保藏法与冷冻干燥保藏法进行保藏。
5. 冷冻保藏法
可分低温冰箱(-20-30℃,-50-80℃)、干冰酒精快速冻结(约-70℃)和液氮(-196℃)等保藏法。
6. 冷冻干燥保藏法
先使微生物在极低温度(-70℃左右)下快速冷冻,然后在减压下利用升华现象除去水分(真空干燥)。有些方法如滤纸保藏法、液氮保藏法和冷冻干燥保藏法等均需使用保护剂来制备细胞悬液,以防止因冷冻或水分不断升华对细胞的损害。保护性溶质可通过氢和离子键对水和细胞所产生的亲和力来稳定细胞成分的构型。保护剂有牛乳、血清、糖类、甘油、二甲亚砜等。
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服务保障:我们拥有专用的技术指导团队,对每售出的每件产品,提供全面的售前、售中和售后服务。保证全网价。
YOR127W BY4742, mating type alpha [12383]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
genomic DNA from strain AmMS 225 (ATCC 66027)Candida albicans (Robin) Berkhout
YNL268W BY4743, heterozygous diploid [21176]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
SS 35Phomopsis viticola (Saccardo) Saccardo
RWK 1538Agaricus bisporus (Lange) Imbach, teleomorph
YKR013W BY4743, homozygous diploid [35084]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
Genomic DNA from Porphyromonas endodontalis ..Porphyromonas endodontalis (van Steenbergen ..
VKM F-1845 [CBS 288.76]Phoma glomerata (Corda) Wollenweber et Hoch ..
NRRL Y-7292 [CBS 4377, IAC 527-64, IFO 1750]Endomycopsella vini (Kreger-van Rij) von Ar ..
pB1-14Mus musculus, mouse
YPR071W BY4743, homozygous diploid [35490]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
PBTreponema saccharophilum Paster and Canale- ..
YKL138C BY4743, homozygous diploid [34988]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
YBR183W BY4741, mating type a [3323]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
Spleen necrosis virus (type C)frozen
A-161Cenococcum graniforme (Sowerby) Ferdinand e ..
TRTC 51884Lasiobolidium orbiculoides Malloch et Benny
NCIB 8619 [L. 930]Acetobacter pasteurianus subsp.pasteurian ..
CFSF 281 [CRFQ 650, FSC-281]Pholiota squarrosa (Persoon : Fries) Quelet
YFR040W BY4742, mating type alpha [15854]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
AB2450Lautropia mirabilis Gerner-Smidt et al.
yWXD4631Homo sapiens, human
Jan-94Curtobacterium flaccumfaciens (Hedges) Coll ..
ISP 5076 [CBS 561.68, ETH 31543, ETH 31544, ..Streptomyces roseus (Krainsky) Pridham et a ..
Hybridoma cell line LIV-1.2982.4A12.1E8.1C4frozen
p423 CYC1freeze-dried
NCTC 6676 [Harke Ram]Salmonella enterica subsp.enterica (e ..
Arabia MountainAcanthamoeba polyphaga (Puschkarew) Page
AL21Mycosphaerella dearnessii Barr, teleomorph
YPL127C BY4742, mating type alpha [12125]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
YGL202W BY4743, homozygous diploid [34569]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
YKL072W BY4741, mating type a [4921]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
YOL105C BY4742, mating type alpha [16255]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
Pneumococcal Polysaccharide Powder Type 7F ( ..frozen 2 mg
GH3 [OCM 47]Methanobacterium sp.
Vaccinia virus ts mutantfrozen
IMRU 1274 [IP 733]Streptomyces somaliensis (Brumpt) Waksman a ..
STX670-12ASaccharomyces cerevisiae Meyen ex E.C. Hans ..
MTF1098Mus musculus, mouse
PFC-082Nectria ditissima Tulasne, teleomorph
大秃马勃使用说明书LRA 85.10.75Candida glabrata (Anderson) Meyer et Yarrow
Soybean Seed S040121
B-285Nocardia canicruria (Bisset and Moore) Adam ..
3/75 IBSuillus variegatus (Sow : Fries) Kuntze
yWXD4593Homo sapiens, human
YGS37Saccharomyces cerevisiae Meyen ex E.C. Hans ..
A-36sRhodovulum tesquicola
Pichia stipitis, NPw9-117Bfrozen
P24Homo sapiens, human
S9/S21 [S21, S9-Y, lambda-S21]Homo sapiens, human
Plasmid pEXJT3T7-hSNORF25frozen
YBL061C BY4743, heterozygous diploid [23087]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
pINCY-based plasmid, DNA59838-1462frozen
stock d4-76Paramecium tetraurelia Sonneborn
BL29-91Homo sapiens, human
yWXD3970Homo sapiens, human
LY 1424 [CBS 6065, NRRL Y-7503]Zygoascus tannicolus (Jacob) Kurtzman et Ro ..
TMC 1305 [P-30; Pierson]Mycobacterium scrofulaceum Prissick and Mas ..
YER035W BY4741, mating type a [167]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
632 [Baumann 218]Pseudomonas stutzeri (Lehmann and Neumann) ..
452 (Ruggles) (Mucoid)Pseudomonas aeruginosa (Schroeter) Migula
ns ..