资料简介：

英文名称    Deformed Epidermal Autoregulatory Factor 1   
中文名称    畸形表皮自调节因子1抗体   
规    格  0.1ml  0.2ml     
研究域    细胞生物  细胞周期蛋白  转录调节因子  表观遗传学     
抗体来源    Rabbit   
克隆类型    Polyclonal   
交叉反应    Mouse, Rat, Chicken, Dog, Cow, Horse,    
产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 畸形表皮自调节因子1抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.   
分 子 量    59kDa   
细胞定位    细胞核 细胞浆 分泌型蛋白    
性    状    Lyophilized or Liquid   
浓    度    1mg/1ml   
免 疫 原    KLH conjugated synthetic peptide derived from human Deformed Epidermal Autoregulatory Factor 1   
亚    型    IgG   
纯化方法    affinity purified by Protein A   
储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4   
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.   

畸形表皮自调节因子1抗体产品介绍    background:
Suppressin, also known as DEAF1 is a transcription factor required for embryonic development. Suppressin contains one SAND domain ａnd one C-terminal MYND-type zinc finger. It interacts with LMO4 ａnd CLIM-2, suggesting that it plays a role mediating cell fate ａnd embryonic pattern formation.

Suppressin is expressed in a variety of tissues ａnd localizes to the nucleus. Several isoforms exist due to alternative splicing and, depending on the isoform, Suppressin is secreted in some cell types. Secreted Suppressin can function to inhibit cell proliferation, arresting cells in the G0 or G1 phase. Mutations in the gene encoding Suppressin may result in a growth advantage leading to the development ａnd progression of neoplasia. This suggest that Supressin is a potential target for cancer therapy.

Function:
Transcription factor that binds to sequence with multiple copies of 5＇-TTC[CG]G-3＇ present in its own promoter ａnd that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5＇-AGGGTTCACCGAAAGTTCA-3＇. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure ａnd skeletal patterning. Regulates epithelial cell proliferation ａnd side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 ａnd retaining it in the cylasm.

Subunit:
Homodimer (Probable). Isoform 1 ａnd isoform 4 may form a heterodimer. Binds to LMO4 (via its C-terminus), LMO2 ａnd CLIM2. May interact with the corepressor NCOR.

Subcellular Location:
Isoform 1: Nucleus. Cylasm. Note=Cylasmic in non-mucinous colorectal carcinoma. When expressed alone, localized almost exclusively in the nucleus but, when expressed with isoform 4, nuclear expression decreases to 32% ａnd cylasmic expression increases by 270%.
Isoform 2: Secreted. Note=Secreted in some cell types.
Isoform 3: Secreted. Note=Secreted in some cell types.
Isoform 4: Cylasm. Nucleus. Note=When expressed alone, localizes mainly in the cylasm but, when expressed with isoform 1, nuclear localization is enhanced.

Tissue Specificity:
Expressed in various tissues ａnd cells such as in peripheral mononuclear cells ａnd hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls.