推荐传代比例　1:2-1:4

推荐换液频率　2~3次/周

背景描述　D283 Med细胞是由Friedman等人于1985年建系，源自一名6岁患有神经管细胞瘤有腹腔转移的白人男性小孩的腹水细胞。

年龄（性别）　男；6岁

组织来源　脑组织

细胞类型　肿瘤细胞

肿瘤类型　成髓瘤细胞

生物安全等　1

倍增时间　~52 hours

致瘤性　Yes, in nude mice(The cells form serially transplantable intercranial ａnd subcutaneous tumors.)Yes, in soft agar

基因表达情况　The cells produce tumors in nude mice ａnd the resulting tumors are glutamine synthetase positive; neuron specific enolase positive; glial fibrillary acidic proteins negative; S100 (S-100) protein negative; The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase; the brain isoenzyme of creatine kinase; L-DOPA decarboxylase; bombesin-like immunoreactivity)