shgoy-1280大鼠热休克蛋白60(Hsp-60)ELISA Kit,48T/96T Human Netrin-1,Ntn1 ELISA Kit
shgoy-1281人热休克蛋白糖蛋白96(HSP gp96)ELISA Kit,48T/96T Human regenerating gene Ⅳ,REG-4 ELISA Kit
shgoy-1282小鼠热休克蛋白糖蛋白96(HSP gp96)ELISA Kit,48T/96T human versican/PG-M/PG-350,VS ELISA Kit
shgoy-1283猴子热休克蛋白糖蛋白96(HSP gp96)ELISA Kit,48T/96T human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor epsilon,Nfkbie
shgoy-1284大鼠热休克蛋白糖蛋白96(HSP gp96)ELISA Kit,48T/96T human ADAM metallopeptidase with thrombospondin type 1 motif4,ADAMTS4 ELISA Kit
shgoy-1285牛可溶性白细胞分化抗原86(B7-2/sCD86)ELISA Kit,48T/96T human similar to RIKEN cDNA 4933429F08 gene ELISA Kit
shgoy-1286人可溶性白细胞分化抗原86(B7-2/sCD86)ELISA Kit,48T/96T human progestin and adipoQ receptor family member Ⅶ,PAQR7 ELISA Kit
shgoy-1287小鼠可溶性白细胞分化抗原86(B7-2/sCD86)ELISA Kit,48T/96Thuman serine/cysteine peptidase inhibitor clade A member 3G,Serpina3g ELISA Kit
shgoy-1288大鼠可溶性白细胞分化抗原86(B7-2/sCD86)ELISA Kit,48T/96Thuman similar to RIKEN cDNA A630077B13 gene ELISA Kit
shgoy-1289人缺血修饰白蛋白(IMA)ELISA Kit,48T/96T human similar to RIKEN cDNA 3110050K21 gene ELISA Kit
shgoy-1290小鼠缺血修饰白蛋白(IMA)ELISA Kit,48T/96T human similar to RIKEN cDNA 2610307008 gene ELISA Kit
shgoy-1291人凝血酶受体(TR)ELISA Kit,48T/96T human similar to RIKEN cDNA 2010109K09 gene ELISA Kit
shgoy-1292小鼠凝血酶受体(TR)ELISA Kit,48T/96T human ras homolog gene family,memmber B,RHOB ELISA Kit
shgoy-1293大鼠凝血酶受体(TR)ELISA Kit,48T/96T human hypothetical protein LOC677340 ELISA Kit
shgoy-1294兔子凝血酶受体(TR)ELISA Kit,48T/96T human hypothetical protein LOC433328 ELISA Kit
shgoy-1295蛇毒因子(CVF)ELISA Kit,48T/96T human myeloid differentiation primary response gene 88,MYD88 ELISA Kit
shgoy-1296人抗凝血酶受体(ATR)ELISA Kit,48T/96T human interferon activated gene 205,Ifi205 ELISA Kit
shgoy-1297小鼠抗凝血酶受体(ATR)ELISA Kit,48T/96T human leukocyte immunoglobulin-like receptor subfamily B member 4,LILRB4 ELISA Kit
Anti-NR2A/NMDAR2A /FITC 荧光素标记谷氨酸受体2A抗体(N端)IgG酵母双杂交系统细胞转化试剂盒
Anti-NR2B/NMDAR2B /FITC 荧光素标记谷氨酸受体2B抗体IgG原生质酵母细胞转化试剂盒
杂交瘤细胞株;Anti-Clas McAb2规格Anti-NR2A/NMDAR2A/FITC 荧光素标记谷氨酸受体2A抗体IgG电击法酵母细胞转化试剂盒
Anti-Phospho-NMDAR2A (Tyr1246) /FITC 荧光素标记磷酸化谷氨酸受体2A抗体IgG酵母菌落直接PCR检测试剂盒
Anti-Phospho-NMDAR2A (Tyr1325) /FITC 荧光素标记磷酸化谷氨酸受体2A抗体IgG酵母细胞溶解试剂盒
Anti-Phospho-NMDAR2B (Tyr1070) /FITC 荧光素标记磷酸化谷氨酸受体2B抗体IgG酵母细胞冻存试剂盒
Anti-Phospho-NMDAR2B (Tyr1472) /FITC 荧光素标记磷酸化谷氨酸受体2B抗体IgG酵母菌YPD培养基
Anti-NR2C/NMDAR2C /FITC 荧光素标记谷氨酸受体2C抗体IgG酵母菌YPD培养基
Anti-NR2D/NMDAR2D /FITC 荧光素标记谷氨酸受体2D抗体IgG酵母菌DOB培养基
Anti-N-ras/FITC 荧光素标记原癌基因抗体IgG酵母菌DOBA培养基
Anti-NRF-1/FITC 荧光素标记核呼吸因子-1抗体IgG酵母菌YNB培养基
Anti-Nrf2/FITC 荧光素标记核因子2相关因子2抗体IgG酵母菌YNBA培养基
Anti-p-Nrf2/FITC 荧光素标记磷酸化核因子2相关因子2抗体IgG酵母菌SD-G培养基
Anti-Nrn1/Cpg15/FITC 荧光素标记转化神经突起蛋白抗体IgG10倍酵母菌SD-G培养基
Anti-CD303/BDCA-2/CLEC4C 荧光素标记C型凝集素结构域家族4成员C抗体IgG酵母菌SD-GAL/RAF培养基
Anti-CD304/BDCA-4/NRP-1 /FITC 荧光素标记神经纤毛蛋白-1抗体IgG10倍酵母菌SD-GAL/RAF培养基
Anti-NRP-2/FITC 荧光素标记神经纤毛蛋白-2抗体IgG酵母菌SD-LEU/TRP培养基
Anti-NEDD4L/NEDD4-2/FITC 荧光素标记泛素连接酶NEDD4-2抗体IgG10倍酵母菌SD-LEU/TRP培养基
1)贴壁细胞传代:提前将培养基、PBS放入37℃水浴锅内预热,用75%酒精擦拭后再放入超净台内,吸除或倒掉细胞瓶内旧培养液,加少量PBS润洗细胞,加入适量胰酶,使胰酶的量能盖住细胞,37℃孵育,每隔2~3min显微镜下观察,待贴壁细胞间间隙变大、细胞趋于圆形但还未漂起时弃去胰酶,加入新鲜培养基,晃动细胞瓶,终止胰酶作用,用吸管小心吹打贴壁的细胞,制成细胞悬液。控制吹打的力度,避免产生大量的气泡,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养,隔天观察贴壁生长情况。
2)悬浮细胞传代:将细胞悬液转移到无菌离心管内,1000rpm离心5min,弃去上清,加入新鲜的培养基,用吸管小心吹散沉淀,制成细胞悬液,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养。