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H2DCFDA

CAS No. : 4091-99-0

MCE 站：H2DCFDA

产品活性：H2DCFDA (DCFH-DA) 是一种可渗透细胞的，用于检测细胞内活性氧 (ROS) 的探针。

研究领域：Immunology/Inflammation  |  NF-κB  |  Metabolic Enzyme/Protease

作用靶点：Reactive Oxygen Species

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions ａnd further diluted before use.
2. Cells are incubated with 5 µM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, ａnd immediately analyzed with flow cytometer.
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA.

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