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H2DCFDA
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H2DCFDA
CAS No. : 4091-99-0
MCE 站:H2DCFDA
产品活性:H2DCFDA (DCFH-DA) 是一种可渗透细胞的,用于检测细胞内活性氧 (ROS) 的探针。
研究领域:Immunology/Inflammation | NF-κB | Metabolic Enzyme/Protease
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
2. Cells are incubated with 5 µM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer.
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA.
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