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Coppersensor 1

CAS No. : 874748-20-6

MCE 站：Coppersensor 1

产品活性：Coppersensor-1 (CS1) 是一种基于硼二吡咯甲烷 (BODIPY) 的荧光传感器，用于选择性和灵敏地检测生物样品(包括活细胞)中的铜 (I) 离子 (Cu⁺)。

研究领域：Others

作用靶点：Others

In Vitro: Coppersensor 1 (CS1) comprising a boron dipyrromethene (BODIPY) chromophore coupled to a thioether-rich receptor, has a picomolar affinity for Cu+ with high selectivity over competing cellular metal ions. CS1 fluorescence increases up to 10-fold on binding to Cu⁺.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs). 1. Coppersensor 1 (CS1) is dissolved in DMSO to obtain a 1 mM stock solution ａnd further diluted before use. ^{2. cells are growing in medium, adding 10 mM CuCl2 stock solution to give a final copper concentration of 100 μM, ａnd incubating for 1-8 h. 3. Adding competing chelator (N-ethyl-3,6,12,15-tetrathia-9-monoazaheptadecane; 100 mM) to copper-supplemented cells to give a final chelator concentration of 500 μM. Mix thoroughly, ａnd incubate in the dark for 5 min at 25°C (A 5- to 10-fold excess of chelator over copper supplementation gives optimal results). 4. Cells are incubated with 1 mM Coppersensor 1 solution to give a final dye concentration of 5 mM. Mix thoroughly. (Higher concentrations of dye may result in high levels of background fluorescence.) Incubating for 5-20 min in the dark at 25°C or 37°C. 5. CS1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal ａnd multiphoton. For standard confocal experiments, best results are obtained with 543 nm excitation to match the absorption maximum of the apo ａnd Cu+-bound probe.}

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