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Cell Cycle ａnd Apoptosis Analysis Kit (PI staining)

MCE 站：Cell Cycle ａnd Apoptosis Analysis Kit (PI staining)

MCE 细胞周期与细胞凋亡检测试剂盒采用碘化丙啶（Propidium Iodide, PI）染色的方法检测细胞周期和细胞凋亡。

Description & Advantages:

MCE Cell Cycle ａnd Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle ａnd cell apoptosis. The content of DNA is changed with the process of cell cycle. DNA can be stained by fluorescent dye, such as Propidium Iodide (PI), to measure its intensity by flow cytometry to monitor the cell cycle distribution in G1/S/G2/M phase as well as apoptosis cells with signals at sub-G1 region. PI is a fluorescent intercalating agent ａnd it binds to DNA by intercalating between the bases with little or no sequence preference. However, PI also binds to RNA, RNase A provided in this kit can eliminate RNA interference. In addition, light scattering also changes during apoptosis. In early apoptosis, light signals from Forward Scatter (FSC) become weak ａnd signals from Side Scatter (SSC) increase or remain unchanged. When cells proceed to the phase of late apoptosis, signals from FSC/SSC both decrease.

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