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Cytotoxicity LDH Assay Kit

MCE 站：Cytotoxicity LDH Assay Kit

MCE LDH Assay Kit 是通过分析释放到培养上清液中的 LDH 活性而测定细胞损伤的试剂盒。配合使用 CCK-8 (HY-K0301) 可获得死细胞、活细胞数据。

Description & Advantages:

The loss of cell membrane integrity due to cell death or membrane damage results in release of certain soluble, cytosolic enzymes. Therefore, acute cytotoxicity can be measured by measuring activity of these enzymes released into the culture supernatant. Lactate dehydragenase (LDH) is a stable enzyme, present in all cell types, ａnd rapidly released into the cell medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

LDH oxidizes lactate to generate NADH, which then reacts with certain dye to generate yellow color. The intensity of the generated color correlates directly with the cell number lysed, which is indicative of cytotoxicity. LDH activity can be easily quantified by spectrophotometer or plate reader at 490 nm.

Fig 1. Schematic of MCE Cytotoxicity LDH Assay Kit.

MCE Cytotoxicity LDH Assay Kit can be directly added to cells to detect LDH activity in the culture supernatant (one-step, direct method). Or you can separate the culture supernatant from cell ａnd use this kit to detect LDH activity (indirect method). The separated cells can be used for other experiments.

Fig 2. Detect LDH activity by direct ａnd indirect method.

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