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5-Carboxyfluorescein diacetate N-succinimidyl ester

CAS No. : 150206-05-6

MCE 站：5-Carboxyfluorescein diacetate N-succinimidyl ester

产品活性：5-Carboxyfluorescein diacetate N-succinimidyl ester 是一种细胞渗透性染料 (Ex=492 nm，Em=517 nm)。5-Carboxyfluorescein diacetate N-succinimidyl ester 可与细胞内分子共价结合，从而标记细胞。5-Carboxyfluorescein diacetate N-succinimidyl ester 可用于示踪淋巴细胞的迁移和增殖。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
1. Dilute the stock solution in PBS (to give a 50 μM solution).
2. Add 110 μL of this solution per milliliter of cells (to give a final concentration of 5 μM) ａnd mix rapidly.
3. After 5 min at room temperature, add 10 vol of PBS containing 5% FBS.
4. Centrifuge cells 5 min at 300 ×g, 20 ℃.
5. Remove the supernatant, ａnd wash threetimes, each time by resuspending in 10 vol PBS containing 5% FBS.
6. Centrifuge cells 5 min at 300 × g, 20 ℃, ａnd removing the supernatant.
Note: Labeling occurs rapidly, ａnd it is essential that the tracker is dispersed as evenly ａnd quickly as possible so that cells are uniformly labeled.

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