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N-Acetyl-DL-phenylalanine β-naphthyl ester

CAS No. : 20874-31-1

MCE 站：N-Acetyl-DL-phenylalanine β-naphthyl ester

产品活性：N-Acetyl-DL-phenylalanine β-naphthyl ester 是一种芳香族氨基酸酯，可作为糜蛋白酶和微生物丝氨酸蛋白酶 (如枯草杆菌蛋白酶) 的显色底物。

研究领域：Others

作用靶点：Others

In Vitro: N-Acetyl-DL-phenylalanine β-naphthyl ester is hydrolyzed in the body by the esterase (APNEase) catalytic action at neutral pHs. APNEase level involves with many muscle wasting conditions suggest the possibility that it may be involved in the turnover ａnd pathological breakdown of muscle proteins.
N-Acetyl-DL-phenylalanine β-naphthyl ester (0.75 mM, DMF; 3 min) as substrate ａnd o-Dianisidine tetrazotized (oD) as the dye, allow the assessment of protease inhibitory activity directly from the yeast P. pastoris expression media.
N-Acetyl-DL-phenylalanine β-naphthyl ester (0.75 mM, DMF; 3 min) (2.4 g/L) provides a visualization result with stained agar gel via the diazo coupling of the β-naphtol produced by the enzymatic hydrolysis of N-Acetyl-DL-phenylalanine β-naphthyl ester (APNE). Circular zones containing inhibitor-proteinase complexes remain coloress while the region containing only proteinase shows a bright pink-purple color.
N-Acetyl-DL-phenylalanine β-naphthyl ester (5 mg/40 mL) has application: determine the class of peptidase in mouse plasma. The enzyme was displayed by immunoprecipitation with antiserum in radial immunodiffusion. After removal of non-precipitated serum ａnd other constituents by washing in excess saline, individual rings of immunoprecipitate were incubated in a solution of a protease inhibitor, followed by washing ａnd staining with the chromogenic substrate (NAPBNE ａnd fast blue B), the picture can be photographed over direct lighting.

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