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2× 高保真 PCR 预混液

MCE 站：2× 高保真 PCR 预混液

2× High-Fidelity PCR Master Mix 是一款新型高保真高效率的即用型 PCR 扩增预混液，适用于高难度长片段的 PCR 扩增实验。

Description & Advantages:

MCE 2× High-Fidelity PCR Master Mix is a new high-fidelity PCR amplification premix. The product contains a new type of modified high-fidelity DNA Polymerase with higher 5’→3’polymerase activity ａnd higher 3’→5’exonuclease activity. The additional hot-start factor can be activated to improve specificity ａnd stability of PCR amplification by minimizing the non-specific amplification ａnd enzyme activity loss. The product also contains a unique Extension-Enhancement factor, making the extending speed ａnd the amplification efficient significantly improved. It can effectively amplify the fragments up to 10 kb, ａnd the amplification speed can reach 15 sec/kb.

MCE 2× High-Fidelity PCR Master Mix is a ready-to-use premix. With the primers ａnd template added, the optimized system will provide sensitive ａnd reliable DNA synthesis. It saves preparation time, reduces the risk of contamination from multiple pipetting steps, ａnd provides consistent reaction-to-reaction performance. This product has no DNA loading buffer, the DNA loading buffer should be added to the PCR product when run on the agarose gel. The PCR product is blunt-ended.

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