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Mucicarmine

CAS No. : 51395-97-2

MCE 站：Mucicarmine

产品活性：Mucicarmine 可用于组织切片中酸性粘多糖 (acid mucopolysaccharides) 的组织学可视化。Mucicarmine 可识别粘蛋白 (mucin) (深玫瑰红色)，可用于鉴别粘蛋白阳性的癌症 (如肝癌)。

研究领域：Others

作用靶点：Others

In Vitro: Mucicarmine successfully stains the presence of mucin on frozen-tissue sections (Mucin stained deep rose).
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
1. Mount the cryostat sections (thickness: 4 to 5 μm) onto coated glass slides.
2. Fix the sections in frozen-section fixative immediately for 30 seconds ａnd then rinse with water for 5 seconds.
3. Immerse the glass slides for 30 seconds into Harris hematoxylin, then consecutively into distilled water, ammonium hydroxide, ａnd distilled water, for 5 seconds in each solution.
4. The mucicarmine stain is placed in the microwave oven ａnd microwave until the solution is about to bubble (30 seconds).
5. Immerse the glass slides in the warmed mucicarmine solution for 2 minutes ａnd rinse slides in distilled water for 5 seconds.
7. Immerse the glass slides consecutively in 95% alcohol, 100% alcohol, ａnd xylene for 5 seconds in each solution, then coverslip the slides.
Note: Nuclei stain (blue) ａnd connective tissue stain (green) maybe is necessary according to your specific needs.

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