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CellTracker Blue CMF2HC Dye
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CellTracker Blue CMF2HC Dye
CAS No. : 215868-45-4
MCE 站:CellTracker Blue CMF2HC Dye
产品活性:CellTracker Blue CMF2HC Dye 是一种蓝色荧光染料,可应用于双通道核酸测序,使用蓝色和紫色激发光 (450-460 nm/400-405nm 或 415-450 nm/480-525nm)。CellTracker Blue CMF2HC Dye 也可用于微生物抗生素敏感性的快速测定。
研究领域:Others
作用靶点:Others
In Vitro: CellTracker Blue CMF2HC Dye is a blue dye that can be excitable by a blue light source having a wavelength of about 450-460 nm, is used as the first or the second detectable label described herein.
Blue/Violet Two-Channel Sequencing Methods:
1.Contacting a primer polynucleotide/taiget polynucleotide complex with a mixture comprising one or more of a first type of nucleotide, a second type of nucleotide, a third type of nucleotide, and a fourth type of nucleotide, wherein the primer polynucleotide is complementary to at least a portion ofthe single stranded target polynucleotide;
2.Incorporating one type of nucleotide from the mixture to the primer polynucleotide to produce an extended primer polynucleotide (i.e., an extended primer polynucleotide/target polynucleotide complex);
3.Performing a first imaging event using a first excitation light source and collecting a first emission signal from the extended primer polynucleotide/target polynucleotide complex with a first emission filer;
4.Performing a second imaging event using a second excitation light source and collecting a second emission signal from the extended primer polynucleotide/taiget polynucleotide complex with a second emission filter;
Note:
a. one of the first excitation light source and the second excitation light source has a wavelength of about 350 nm to about 410 nm, and the other one of the first excitation light source and the second excitation light source has a wavelength of about 450 nm to about 460 nm;
b. one of the first emission filter and the second emission filter has a detection wavelength of about 415 nm to about 450 nm, and the other one of the first emission filter and the second emission filter has a detection wavelength of about 480 nm to about 525 nm.
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