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Uniblue A sodium

CAS No. : 14541-90-3

MCE 站：Uniblue A sodium

产品活性：Uniblue A sodium 是一种反应性蛋白质染料，可用于蛋白质的共价 pre-gel 染色 (Ex=594 nM)。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
1. Prepare the derivatization buffer: 100 mM NaHCO₃, 10% SDS, pH 8-9.
2. Add 10 L of 200 mM Uniblue A solution (dissolved in derivatization buffer) to 90 L protein solution.
3. The samples are heated at 100°C for 1 min to perform the staining.
4. Add 100 L of reducing solution composed of 10% glycerol ａnd 20 mM dithiotreitol (DTT) in 200 mM Tris buffer with pH 6.8.
5. Excess Uniblue A reacts with Tris, resulting in a blue compound, serving as running front indicator in electrophoresis.
6. The sample is heated another minute at 100°C in order to achieve efficient reduction ａnd allowed to cool to room temperature.
7. Add 20 L alkylation solution containing 550 mM iodoacetamide (IAA), incubated for 5 minutes.
8. the samples are subjected to SDS-PAGE.
Note: Dry protein samples or samples in compatible buffers (i.e. free of amines) can be diluted directly with the derivatization buffer solution to 5 mg/mL. In other cases, a prior trichloroacetic acid (TCA)/acetone precipitation or buffer exchange by ultrafiltration is recommended (see below).
As positive control, bovine serum albumin (BSA) in derivatization buffer is used at a concentration of 10 mg/mL.

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