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BODIPY TR methyl ester

CAS No. : 150152-63-9

MCE 站：BODIPY TR methyl ester

产品活性：BODIPY TR methyl ester 是一种 GFP 亲脂性复染剂。BODIPY TR methyl ester 很容易渗透细胞膜，并在膜内细胞器中定位，但不强烈地定位于质膜中。BODIPY TR methyl ester 是一种较好的红色荧光活体染色剂 (Ex=568 nm, Em=625 nm)，可用于揭示细胞核的位置和形状、胚胎组织内细胞的形状，以及整个胚胎内器官形成组织的边界。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
Vital staining procedures:
1. Solubilized into anhydrous DMSO as a 5 mM stock solution.
2. The stock DMSO solution is diluted 1:50 into Embryo Rearing Medium (ERM) buffered with 5 mM HEPES (pH 7.2), making a final labeling solution of 100 M BODIPY TR methyl ester dye with 2% DMSO. Stored at -20°C.
3. Embryos are stained in 100 M BODIPY TR methyl ester dye for 1 h, then passed through three successive washes in HEPES-buffered ERM.
4. Embryos are mounted in an openfaced chamber for imaging. For time-lapse recordings, vitally stained embryos were deyolked ａnd secured to a coverslip.
5. Embryos used for fixation experiments were fixed in 4% paraformaldehyde in HEPES-buffered ERM at 4°C for 1 h, before being washed in ERM ａnd mounted for confocal imaging.
6. Analyze sample on confocal microscope (with a 640 × 480 pixel frame buffer ａnd an air-cooled Ar-Kr laser), coexciting GFP ａnd BODIPY TR methyl ester dye with two wavelengths of the major Ar-Kr laser lines (488 ａnd 568 nm) simultaneously.

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