MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

Toluidine Blue (purity 36%)

CAS No. :

MCE 站：Toluidine Blue (purity 36%)

产品活性：Toluidine Blue (Toluidine Blue O) purity 36% 是一种碱性的醌亚胺染料 (活体染料)，对酸性组织成分有很高的亲和力，可将细胞核染成蓝色，多糖染成紫色。Toluidine Blue purity 36% 对肥大细胞、粘蛋白和软骨细胞显示异染性。Toluidine Blue purity 36% 可使植物组织和细胞的不同成分染成不同的颜色。Toluidine Blue purity 36% 还可作为辅助诊断工具，鉴别恶性病变，如癌症。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
Detection of infiltrating Mast cells:
1. Tissue processing
1)Immersed tissue samples in 10% buffered formalin for 24 h.
2)Remove tissues from the fixative ａnd place in 70% alcohol.
3)Embed in paraffin: sequentially incubate in 70, 85, 95, ａnd 100% ethanol, xylene, ａnd molten paraffin (30 min incubation each), ａnd set tissues in paraffin blocks.
4)Cut paraffin-embedded tissue in 5-7 μm sections ａnd place onto glass slides.
5)Bake the slides for a minimum of 1 h at 55°C before use.
2. Tissue staining
1)De-wax paraffin-embedded tissue sections by immersing slides 5 min in xylene (or Histo-Clear), ａnd rehydrate tissue by sequential immersions (5 min each) in 100%, 95%, ａnd 70% ethanol ａnd a final 2 min immersion in distilled water.
2)Place the slides in Harris’ hematoxylin solution for 70 s.
3)Briefly wash the excess of hematoxylin by immersing the slides two or three times in clean tap water. Briefly tap the slides on paper towels to remove excess water.
4)Immerse slides briefly three times in 70% alcohol, ａnd wash again in clean tap water for 3 min. Remove excess water.
5)Place slides in Scott’s bluing solution for up to a minute. This step gently transforms hematoxylin into an insoluble blue color within the nucleus.
6)Inspect the slides under the bright-light microscope to ensure the nuclei are properly stained.
7)Wash slides in clean tap water for 3 min, ａnd remove excess water.
8)Immerse slides in clean ~1.1% Toluidine Blue purity 36% solution for 4 min (Note 1).
9)Wash slides in clean tap water for 3 min ａnd remove excess water.
10)Quickly dip the slides three times in one batch of 70% ethanol ａnd then dip them five times in 5% eosin solution.
11)Dehydrate the slides by successive immersion in 70% (2 min), 95% (twice, 2 min each), ａnd 100% ethanol (twice, 2 min each); conclude this step by immersing the slides in xylene (or Histo-Clear) (twice, 3 min each).
12)Permanently mount each slide with a clean coverslip using xylene-based mounting medium.
13)Let slides dry for a minimum of 60 min before analyzing them under the bright-light microscope.
Note 1: 1.1% Toluidine Blue purity 36% solution: mix 1.1 g Toluidine Blue purity 36% with 100 mL 0.1 M sodium acetate buffer pH 4. Stir well. Adjust the pH to 2.0-2.5 by adding drops of 1 M hydrochloric acid. Staining is performed at 25 ℃, protected from light. Ready to use.
Application of Toluidine Blue purity 36%:
1. Connective tissue mucins, especially acid mucins. The tissue stains purple to red, while the background is stained blue.
2. Mast cell granules stain purple in color due to the presence of heparin ａnd histamine.
3. Amyloid stain blue but under polarized light they give a bright red birefringence.
4. Endocrine cell granules are stained purple to red (concentration of stain is 0.01%).
5. Sulfatides stain red brown or yellow. Only lipids that are sufficiently acidic to induce a metachromatic shift are stained.
6. Corneybacterium diphtheria contains granules with polymerized inorganic polyphosphate, which stains red violet color.
7. Helicobacter stains dark blue against a variably blue background (concentration of stain is 1%).
8. Toluidine Blue purity 36% can be used to stain frozen section because of the rapidity of the staining procedure (10-20 s) ａnd better clarity of the cells.9. Toluidine Blue purity 36% can also dye the lignins of the plant blue-green, the phloem blue-purple, ａnd the rest of the plant pale blue-green.

相关产品：Antitumor agent-67  |  MeOSuc-Ala-Ala-Pro-Val-pNA  |  BDP 558/568 amine  |  ATR-IN-9  |  TBTDC  |  (S)-Norfluoxetine-d5 (phenyl-d5)  |  H-4-Pal-OH  |  (1R,2S,7R)-Sitafloxacin-d4 hydrochloride  |  6-Hydroxy-4-methylcoumarin  |  Acridine Orange base  |  CEF19, Epstein-Barr Virus latent NA-3A (458-466)  |  AC-green  |  AMT-NHS  |  Nylidrin hydrochloride  |  Naphthalene-1,2-dicarbaldehyde  |  C13-112-tri-tail  |  (4S)-1-Boc-4-fluoro-D-proline  |  D-(+)-Fucose  |  OVA Peptide 323-339  |  Secretin (swine)  |  Bletilloside A  |  Transcriptional Intermediary Factor 2 (TIF2) (740-753)  |  RPR132595A-phenylmethyl hydrogen carbonate-d3  |  Fmoc-Ser(tBu)-OH-13C3,15N  |  Pep-1 (uncapped)  |  Aspochalasin M  |  Dihydrofuran-3(2H)-one  |  Rhodamine 800  |  (S)-Tamsulosin-d3 hydrochloride  |  BPK-29 hydrochloride

热门产品线：重组蛋白  |  化合物库  |  天然产物  |  染料试剂  |  PROTAC  |  同位素标记物  |  寡核苷酸

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Dye Reagents  |  PROTAC  |  Isotope-Labeled Compounds  |  Oligonucleotides

品牌介绍:
•   MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供*新*全的高品质小分子活性化合物；
•   10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果；
•   专业团队跟踪*新的制药及生命科学研究进展，为您提供全球*新的活性化合物；
•   与世界各大制药公司及知名科研机构建立了长期的合作。