MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

Acridine Orange base

CAS No. : 494-38-2

MCE 站：Acridine Orange base

产品活性：Acridine Orange base 是一种细胞通透性荧光染料，能将生物体 (细菌，寄生虫，病毒等) 染成亮橙色，而当在适当条件下 (pH=3.5, Ex=460 nm) 可将人类细胞区别性地染成绿色，以便用荧光显微镜进行检测。Acridine Orange base 与 dsDNA 结合时发出绿色荧光 (Ex=488, Em=520-524)，与 ssDNA (Ex=457, Em= 630-644) 或 ssRNA 结合时发出红色荧光 (Ex=457, Em=630-644)，也可用于细胞周期测定。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
1. Differential staining of DNA ａnd RNA of unfixed cells:
(1) Set up the flow cytometer with excitation at 488 nm, using emission filters ａnd a dichroic mirror that discriminate green fluorescence (measured at 515-545 nm) ａnd red luminescence (measured preferably above 640 or 650 nm).
(2) Transfer a 0.2-mL aliquot of the original cell suspension to a small glass or plastic tube (e.g., 2- or 5-mL volume). Chill on ice.
(3) Gently add 0.4 mL ice-cold cell permeabilizing solution. Wait 15 s, keeping cells on ice.
(4) Gently add 1.2 mL ice-cold Acridine Orange base staining solution. Keep cells on ice in the dark.
(5) Measure ａnd record cell fluorescence in the flow cytometer during the 2 to 10 min after addition of Acridine Orange base staining solution.
2. Differential staining of fixed cells:
(1a) For cells in suspension culture or hematologic samples: Rinse cells once with ice-cold PBS ａnd suspend in ice-cold PBS at 10⁶ cells/mL.
(1b) For cells attached to tissue culture plates: Collect cells from flasks or petri plates by trypsinization, pool the trypsinized cells with cells floating in the medium (mostly detached mitotic ａnd dead cells), ａnd rinse once with medium containing serum to inactivate the trypsin. Suspend cells in ice-cold PBS at 10⁶ cells/mL.
(1c) For cells isolated from solid tumors: Rinse cells free of any enzyme used for cell dissociation ａnd suspend in ice-cold PBS at 10⁶ cells/mL.
(2) With a Pasteur pipet transfer 1 mL cell suspension to a 15-mL conical glass tube containing 10 mL ice-cold 70% ethanol. Fix cells ≥2 h on ice.
(3) Centrifuge tubes 5 min at 300 × g, 4℃. Remove all ethanol, rinse cells once with ice-cold PBS, ａnd suspend in ice-cold PBS at a density of < 2 × 10⁶ cells/mL.
(4) Withdraw 0.2 mL cell suspension (≤ 2 × 10⁵ cells) ａnd transfer to a small tube (e.g., 2 or 5 mL volume). Chill on ice.
(5) Add 0.4 mL ice-cold permeabilizing solution. Wait 15 s, keeping cells on ice.
(6) Add 1.2 mL ice-cold Acridine Orange base staining solution. Keep cells on ice.
(7) Measure ａnd record cell fluorescence in the flow cytometer during the 2 to 10 min after addition of Acridine Orange base staining solution.

相关产品：Antitumor agent-67  |  MeOSuc-Ala-Ala-Pro-Val-pNA  |  BDP 558/568 amine  |  ATR-IN-9  |  TBTDC  |  (S)-Norfluoxetine-d5 (phenyl-d5)  |  H-4-Pal-OH  |  (1R,2S,7R)-Sitafloxacin-d4 hydrochloride  |  6-Hydroxy-4-methylcoumarin  |  CEF19, Epstein-Barr Virus latent NA-3A (458-466)  |  AC-green  |  AMT-NHS  |  Nylidrin hydrochloride  |  Naphthalene-1,2-dicarbaldehyde  |  C13-112-tri-tail  |  (4S)-1-Boc-4-fluoro-D-proline  |  D-(+)-Fucose  |  OVA Peptide 323-339  |  Secretin (swine)  |  Bletilloside A  |  Transcriptional Intermediary Factor 2 (TIF2) (740-753)  |  RPR132595A-phenylmethyl hydrogen carbonate-d3  |  Fmoc-Ser(tBu)-OH-13C3,15N  |  Pep-1 (uncapped)  |  Aspochalasin M  |  Dihydrofuran-3(2H)-one  |  Rhodamine 800  |  (S)-Tamsulosin-d3 hydrochloride  |  BPK-29 hydrochloride

热门产品线：重组蛋白  |  化合物库  |  天然产物  |  染料试剂  |  PROTAC  |  同位素标记物  |  寡核苷酸

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Dye Reagents  |  PROTAC  |  Isotope-Labeled Compounds  |  Oligonucleotides

品牌介绍:
•   MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供*新*全的高品质小分子活性化合物；
•   10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果；
•   专业团队跟踪*新的制药及生命科学研究进展，为您提供全球*新的活性化合物；
•   与世界各大制药公司及知名科研机构建立了长期的合作。