MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

5-Nitro BAPTA

CAS No. : 124251-83-8

MCE 站：5-Nitro BAPTA

产品活性：5-Nitro BAPTA 是一种钙螯合剂，与 2- Me 取代的 TM 结合 (作为荧光部分)，可形成红色荧光探针 (CaTM-2 AM)，可对培养的活细胞中细胞质 Ca²⁺ 成像。5-Nitro BAPTA 是用于合成 Ca²⁺ 特异性螯合物、Ca²⁺ 缓冲液和荧光 Ca²⁺ 指示剂的基石。

研究领域：Others

作用靶点：Others

In Vitro: 5-Nitro BAPTA, designed to a red fluorescent probe for cytoplasmic Ca²⁺ with strong emission in the long-wavelength region.
General procedure for fluorescence imaging of cultured Hela cells:
1.Plate cells onto a 35-mm poly-L-lysinecoated glass-bottomed dish (Matsunami) in DMEM supplemented with 10% (v/v) fetal bovine serum, 1% penicillin ａnd 1% streptomycin.
2. Remove DMEM, wash the dish with HBSS 3 times, ａnd then add CaTM-2 AM (3 μM) in Hanks’ Balanced Salt Solution (HBSS) containing 0.3% DMSO as a cosolvent.
3. Incubate at 37°C for 30 min, remove medium ａnd wash dishes with HBSS 3 times. The cells can be observed in HBSS.
4. Capture fluorescence images with excitation ａnd emission wavelength of 590/610680 nm.
General procedure for fluorescence imaging of slices:
1. Incubate slide cultures with 2 mL dye solution at 37 C for 40 min. The dye solution is artificial cerebrospinal fluid (aCSF) containing 10 μM CaTM-2 AM, 0.01% Pluronic F-127, ａnd 0.005% Cremophor EL. aCSF consisted of : 126 mM NaCl, 26 mM NaHCO₃, 3.5 mM KCl, 1.24 mM NaH₂PO₄, 1.3 mM MgSO₄, 1.2 mM CaCl₂, ａnd 10 glucose.
2. Wash slieds with aCSF three times ａnd recover in 2 mL aCSF at 37 C for 45 min, during which 2 L of 1 mM Acridine orange was added to the aCSF at time 40 min.
3. Transferre slice cultures into a recording chamber heated at 35 C ａnd continuously perfused with aCSF at 2 mL/min.
4. Acqure images at 10 frames/s with a Nipkowdisk confocal unit (CSUX-1, Yokogawa Electric, Tokyo, Japan), cooled CCD camera (iXon DU897, Andor, Belfast, UK), a water-immersion objective lens (16×, 0. NA, Nikon, Tokyo, Japan), ａnd image acquisition software (Solis, Andor Technology, Belfast, UK).
5. Set the excitation wavelength to 488 nm (7 mW) ａnd 568 nm (15 mW) for Acridine orange ａnd CaTM-2 with an argon-krypton laser (641-YB-A01; Melles Griot, Carlsbad, CA, USA) ａnd set the emission wavelength to 520-535 nm ａnd 617-673 nm band-pass emission filters, respectively.
6. Analysis data with custom-made software written in Microsoft Visual Basic.
7. Calculate fluorescence change ΔF/F as (Ft-F0)/F0, where Ft is the fluorescence intensity at frame time t, ａnd F0 is the average baseline.

相关产品：Antitumor agent-67  |  MeOSuc-Ala-Ala-Pro-Val-pNA  |  BDP 558/568 amine  |  ATR-IN-9  |  TBTDC  |  (S)-Norfluoxetine-d5 (phenyl-d5)  |  H-4-Pal-OH  |  (1R,2S,7R)-Sitafloxacin-d4 hydrochloride  |  6-Hydroxy-4-methylcoumarin  |  Acridine Orange base  |  CEF19, Epstein-Barr Virus latent NA-3A (458-466)  |  AC-green  |  AMT-NHS  |  Nylidrin hydrochloride  |  Naphthalene-1,2-dicarbaldehyde  |  C13-112-tri-tail  |  (4S)-1-Boc-4-fluoro-D-proline  |  D-(+)-Fucose  |  OVA Peptide 323-339  |  Secretin (swine)  |  Bletilloside A  |  Transcriptional Intermediary Factor 2 (TIF2) (740-753)  |  RPR132595A-phenylmethyl hydrogen carbonate-d3  |  Fmoc-Ser(tBu)-OH-13C3,15N  |  Pep-1 (uncapped)  |  Aspochalasin M  |  Dihydrofuran-3(2H)-one  |  Rhodamine 800  |  (S)-Tamsulosin-d3 hydrochloride  |  BPK-29 hydrochloride

热门产品线：重组蛋白  |  化合物库  |  天然产物  |  染料试剂  |  PROTAC  |  同位素标记物  |  寡核苷酸

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Dye Reagents  |  PROTAC  |  Isotope-Labeled Compounds  |  Oligonucleotides

品牌介绍:
•   MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供*新*全的高品质小分子活性化合物；
•   10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果；
•   专业团队跟踪*新的制药及生命科学研究进展，为您提供全球*新的活性化合物；
•   与世界各大制药公司及知名科研机构建立了长期的合作。