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Sulfo-NHS-LC-LC-Biotin

CAS No. : 194041-66-2

MCE 站：Sulfo-NHS-LC-LC-Biotin

产品活性：Sulfo-NHS-LC-LC-Biotin (Biotin-XX-SSE) 是一种生物素试剂，用于标记暴露于完整外泌体外叶的蛋白 (proteins)，在生物素和胺反应连接剂之间包含一个较大的间隔臂。该连接子的大小有助于克服空间位阻，并提高拥挤外泌体表面的标记效率。

研究领域：Others

作用靶点：Others

In Vitro: Biotinylation of exosome proteins: Two hundred micrograms of intact exosomes were mixed with 10mM Sulfo-NHS-LC-LC-Biotin at room temperature for 30min. Four conditions were taken into account during this experiment: (a) an excess of Sulfo-NHS-LC-LC-Biotin was used to favor a complete saturation of exposed lysine residues ａnd potential N-terminus, (b) the presence of the sulfonate group in Sulfo-NHS-LC-LC-Biotin blocks the reagent from penetrating the exosomal membrane, (c) Sulfo-NHS-LC-LC-Biotin has an spacer arm of 30.5 angstroms which improves the biotinylation of proteins in their natural conformation, ａnd (d) amino acids labeled with Sulfo-NHS-LC-LC-Biotin will have an increase in mass of 452Da. After incubation, the excess of Sulfo-NHS-LC-LC-Biotin was removed using a 10KDa MWCO filtration device.
Rat aortic endothelial cells (RAEC) were surface modified in suspension with 1 mM Sulfo-NHS-LC-LC-biotin for 10 min, followed by pelleting ａnd resuspension in PBS.

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