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Tetrabromorhodamine 123 bromide

CAS No. : 623903-26-4

MCE 站：Tetrabromorhodamine 123 bromide

产品活性：Tetrabromorhodamine 123 (TBrRh123) bromide 是一种光敏剂。Tetrabromorhodamine 123 bromide 可用于光动力疗法 (PDT) 和癌症的研究。

研究领域：Others

作用靶点：Others

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
A. Labeling of Cells:
1. Incubate the cells according to your normal protocol.
2. Cells are incubated with DMEM containing 5 μM TBR ａnd maintained in the dark in a CO₂ incubator at 37℃.
3. After 1 h, chang the medium to normal culture medium without phenol red, ａnd cells were exposed to visible light with a 500 W Xe arc with a filter.
B. The intracellular localization of TBR:
1. Cells are cultured in 35 mm diameter glassbottomed dishes for 48 h. Incubate the cells according to your normal protocol.
2. For triple-staining, medium is changed to a solution containing BODIPY-TR ceramide (5 μM).
3. Cells are incubated at 48℃ for 20 min ａnd then further incubated at 37℃ for 1 h, after which the solution is changed to normal medium containing 5 μM TBR.
4. The stock solution (1 mM) of Hoechst 33342 was added to the medium (final concentration, 100 μM), ａnd cells were incubated for 15 min.
5. Cells are washed with phosphate-buffered saline (plus Ca²⁺, Mg²⁺) ａnd then observed with the aid of confocal laser scanning microscopy (LSM).

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