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NIR-βgal-2

CAS No. :

MCE 站：NIR-βgal-2

产品活性：NIR-βgal-2 是一种 β-半乳糖苷酶激活的近红外荧光探针，具有优异的灵敏度。NIR-βgal-2 可用于可视化乳腺癌中的 β-半乳糖苷酶。

研究领域：Others

作用靶点：Others

In Vitro: NIR-βgal-2 displays superior sensitivity (detection limit = 2.0 × 10^-3 U/mL), great affinity (K_m = 1.84 μM), ａnd catalytic efficiency (k_cat/K_m = 0.24 μM^-1 s^-1) for β-galactosidase.
The survival rate of cells (SKOV-3, 4T1, A549, ａnd L02 cells) in the presence of NIR-βgal-2 is higher than 80% after 24 h, suggesting that the probe had low cytotoxicity toward the cells.
Human ovarian carcinoma cells (SKOV-3 cells) ａnd mouse breast cancer cells (4T1 cells) that endogenously overexpress β-galactosidase are chosen as cell models for this study. In comparison, human normal liver cells (L02 cells) ａnd human lung cancer cells (A549 cells) are selected as negative groups. After incubating with NIR-βgal-2 (5 μM) for 30 min, can observe bright fluorescence signals in SKOV-3 ａnd 4T1 cells, whereas faint NIR fluorescence is observed in A549 ａnd L02 cells. NIR-βgal-2 could differentiate efficiently ａnd rapidly detect β-galactosidase in different cells ａnd can distinguish cancer cells that overexpress β-galactosidase.

In Vivo: NIR-βgal-2 (tumor injection; 100 μM/25 μL; 0, 10, 20, 30, ａnd 60 min; Ex=640 nm, Em=695-770 nm) observes an intense signal in the tumor area in 4T1-bearing mice.

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