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Cypate

CAS No. : 95837-47-1

MCE 站：Cypate

产品活性：Cypate 是一种花青染料，是一种用于体内肿瘤成像的近红外 (NIR) 荧光探针。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, ａnd should be modified according to your specific needs).
1.Plated 1×10⁶ of each cell type on individual 35-mm glass-bottom petri dishes.
2.After 24 hours, the cell lines are incubated for 2 hours with a 10 M solution of Cypate in phenol red free medium.
3.After treatment, the cells are either washed 3× with PBS ａnd fixed with 4% paraformaldehyde for imaging or has their intracellular Cypate contents collected for spectroscopy and/or LC-MS analysis.
4.Confocal microscopy is performed on in vitro cell culture experiments. Cypate is excited with 647 nm wavelength.

In Vivo: Cypate (10 nmol; IV; every 24 hours for 6 days) causes the liver to have the highest accumulation.
The fluorescence signal of Cypate (5 mg/kg; IV) in the tumor of mice (Balb/c mice with 4T1 cells) is weak ａnd quickly decayed, possibly due to fast elimination of free cypate from body. Stronger fluorescence in liver ａnd kidney is observed in free cypate group. The Cypate fluorescence signal is assessed using an IVIS Lumina imaging system (Ex-745 nm; Em-800 nm).

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