当前位置: 易推广 > 生物试剂/抗体/细胞 > 生化试剂 > 其他 > MedChemExpress > 产品展示 > MCE信号通路 > 其他 > AF488 NHS ester
AF488 NHS ester
企业档案
会员类型:会员
已获得易推广信誉 等级评定
(0 -40)基础信誉积累,可浏览访问
(41-90)良好信誉积累,可接洽商谈
(91+ )优质信誉积累,可持续信赖
易推广会员:8年
最后认证时间:
注册号: 【已认证】
法人代表: 【已认证】
企业类型:生产商 【已认证】
注册资金:人民币万 【已认证】
产品数:91196
参观次数:10609832
联系我们
MedChemExpress
电话:021-58955995
联系人:客服部 (请说明是在易推广看到的!谢谢)
手机:13611715263
邮编:201203
邮箱:sales@medchemexpress.cn
地址:上海上海
详细内容
MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。
AF488 NHS ester
CAS No. : 1374019-99-4
MCE 站:AF488 NHS ester
产品活性:AF488 NHS ester 是一种胺特异性荧光探针 (Em=525 nm)。AF488 NHS ester 在水和生物样品中,与巯基和胺反应,并在衍生化后改变它们的化学结构和荧光性质。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: IgG fluorescent labeling and anti-IgG solid phase peptide libraries screening of AF488 NHS ester
(1) Dissolve the lyophilized immunoglobulin in a solution of 50 mM sodium phosphate, 20 mM sodium chloride and PH 8.3 at a concentration of 5 g/L.
(2) Dissolve 1 mg of AF488 NHS ester in 100 L extra dry DMF, add 1 mL of solution in (1), and incubate at room temperature for 1 h.
(3) Collect samples with Amicon Ultra 0.5-mL Centrifugal Filter Unit equipped with 3-kDa MWCO filters.
(4) The deprotected libraries of hexameric or tetrameric are washed in 50 mM sodium phosphate, 150 mM sodium chloride, PH 7.4 (PBS) solution with 5× settled resin volume washing three times to balance.
(5) Dilute IgG-AF488 with 50 mM sodium phosphate, 150 mM sodium chloride, 0.2% Tween (PH 7.4) solution until the final concentration is 1.3 mg/mL.
(6) Incubate (4) and (5) at 2-8°C overnight.
(7) Wash resin beads with 50 mM sodium phosphate, 150 mM sodium chloride, 0.1% Tween 20, PH 7.4 (PBS-T).
(8) The resin is deposited in a 96-well plate of 40 L PBS-T with one bead per well, and then the fluorescence microscope is used to image at 10× magnification. Under 480 nm excitation, Alexa Fluor 488 fluorescence measurement and fluorescence screening are carried out with 510 nm emission intensity as the threshold.
AF488 NHS ester storage solution
Prepare 20 mM AF488 NHS ester with DMF.
Note: AF488 NHS ester storage solution is recommended to be stored in dark at -20°C after sub-packaging.
相关产品:MeOSuc-Ala-Ala-Pro-Val-pNA | BDP 558/568 amine | AC-green | Naphthalene-1,2-dicarbaldehyde | Rhodamine 800 | Acridine Orange hydrochloride | N-(7-Nitrobenzofurazan-4-yl)phallacidin | Biotin-11-UTP | BODIPY FL SSE | 1,2-Diamino-3,4-ethylenedioxybenzene | Sulfo-Cyanine5.5 NHS ester tripotassium | BODIPY 505/515 | 3-Acetylumbelliferyl β-D-Glucopyranoside | Ac-DNLD-AMC | Mucicarmine | 7-Aminocoumarin | NEP | Sulfo-cyanine3 azide sodium | Dihydrorhodamine 123 | N-Acryloyl-1-pyrenebutylamine | Dabcyl acid | 7-Diethylamino-4-methylcoumarin | 9-(2-Carboxy-2-cyanovinyl)julolidine | RT-NH2 | Coumberol | Carmoisine | 4-Methylumbelliferyl-N-acetyl-α-D-glucosaminide | MitoMark Red I | PA Janelia Fluor 549, SE | BODIPY FL C5-Ceramide
热门产品线:重组蛋白 | 化合物库 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物 | 寡核苷酸
Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Fluorescent Dye | PROTAC | Isotope-Labeled Compounds | Oligonucleotides
品牌介绍:
• MCE (MedChemExpress) 拥有200 多种全球独家化合物库,我们致力于为全球科研客户提供前沿*全的高品质小分子活性化合物;
• 50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领;
• 产品种类涵盖各种重组蛋白,多肽,常用试剂盒 ,更有 PROTAC、ADC 等特色产品,广泛应用于新药研发、生命科学等科研项目;
• 提供虚拟筛选,离子通道筛选,代谢组学分析检测分析,药物筛选等专业技术服务;
• 设有专业的实验中心和严格的质控、验证体系;
• 提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告,确保产品的高纯度、高品质;
• 产品的生物活性多经各国客户实验验证;
• Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果;
• 专业团队跟踪*新的制药及生命科学研究进展,为您提供全球*新的活性化合物;
• 与世界各大制药公司及知名科研机构建立了长期的合作。
热门标签:af-488