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MitoTracker Deep Red FM

CAS No. : 873315-86-7

MCE 站：MitoTracker Deep Red FM

产品活性：MitoTracker Deep Red FM?是一种绿色荧光染料，可以选择性地积聚在线粒体基质中。MitoTracker Deep Red FM 通过与半胱氨酸残基的游离硫醇基反应共价结合线粒体蛋白，从而可以不依赖膜电位即对线粒体膜电位进行染色。激发/发射波长 644/665 nm。（储存方式：避光保存。）

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: General Protocol

1. Preparation of MitoTracker Deep Red FM working solution
1.1 Preparation of the stock solution
Dissolve 50 μg MitoTracker Deep Red FM in 74.4 μL DMSO to obtain 1 mM of stock solution.
Note: It is recommended to store the stock solution at -20 °C ａnd -80 °C away from light ａnd avoid repetitive freeze-thaw cycles.
1.2 Preparation of MitoTracker Deep Red FM working solution
Dilute the stock solution in serum-free cell culture medium or PBS dilute at 1:5000 or 1:50000 to obtain 20-200 nM of working solution.
Note: Please adjust the concentration of MitoTracker Deep Red FM working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells（6-well plate）
a. Centrifuge at 1000 g at 4°C for 3-5 minutes ａnd then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL.
b. Add 1 mL of working solution, ａnd then incubate at room temperature for 15-45 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes ａnd then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium ａnd aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

Storage
-20°C, 1 year. Protect from light

Precautions
1. Please adjust the concentration of MitoTracker Deep Red FM working solution according to the actual situation.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety ａnd health, please wear a lab coat ａnd disposable gloves to operate.

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