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GMP IL-12 Protein, Human (HEK293, His)

MCE 站：GMP IL-12 Protein, Human (HEK293, His)

Interleukin-12 subunit alpha (IL-12A; IL-12p35) 是一种免疫抑制细胞因子，编码细胞因子 IL-12 的亚基，作用于 T 细胞和自然杀伤细胞，并具有广泛的生物活性。IL-12A 与IL-12B 异二聚化形成 IL-12 细胞因子或与 EBI3/IL27B 形成 IL-35 细胞因子。IL-12 Protein, Human (HEK293, His)，一种 GMP 级别的重组蛋白，是在 HEK293 细胞中产生的，由 IL-12A (M1-A219) 和 IL-12B (M1-S328) 组成，其 C 末端带有His 标签。

Synonyms: GMP IL-12; Interleukin 12; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40

Species: Human

Source: HEK293

Biological Activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED₅₀ for this effect is typically 0.04-0.4 ng/mL. The specific activity of recombinant human IL-12 is >10000 Units/μg, which is calibrated against the human IL-12 WHO International Standard

研究背景：Interleukin-12 subunit alpha (IL-12A; IL-12p35), an immune-suppressive cytokine, encodes a subunit of the cytokine IL-12 that acts on T ａnd natural killer cells, ａnd has a broad array of biological activities. IL-12A is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, ａnd a 40-kD subunit that is a member of the cytokine receptor family. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells ａnd dendritic cells (DCs) as well as macrophages ａnd granulocytes, induces the production of IFN-gamma, favors the differentiation of Th1 cells ａnd is an important link between innate resistance ａnd adaptive immunity.
The amino acid sequence of human IL-12A protein has low homology between mouse IL-12A protein. While, human IL-12A shares 94% aa sequence identity with Rhesus Macaque IL-12A protein.
IL-12 family cytokines are pleiotropic immunological playmakers that coordinate innate ａnd adaptive immune responses mainly via regulation of T-cell populations. The four core members of the Interleukin-12 (IL-12) family of cytokines, IL-12, IL-23, IL-27 ａnd IL-35 are heterodimers which share α-cytokine subunits (IL-12p35 (IL-12A), IL-23p19, ａnd IL-27p28) ａnd β-cytokine subunits (IL-12p40, Ebi3). The subunits are each encoded by separate chromosomes ａnd their expression is regulated independently. Among them, the IL-12A subunit has immunoregulatory functions hitherto attributed to IL-35. Pairing of the α-subunits, IL-12A or IL-23p19 with IL-12p40, gives rise to the two pro-inflammatory members IL-12 ａnd IL-23, respectively, whereas the two immunosuppressive members of the family, IL-27 ａnd IL-35, derive from pairing of IL-27p28 or IL-12A with Ebi3.
IL-12A suppresses lymphocyte proliferation, induces expansion of IL-10-expressing ａnd IL-35-expressing B cells ａnd ameliorates autoimmune uveitis in mice by antagonizing pathogenic Th17 responses. IL-12A-mediated expansion of Treg ａnd Breg cells ａnd its amelioration of experimental autoimmune encephalomyelitis (EAE) correlated with inhibition of cytokine-induced activation of STAT1/STAT3 pathways. IL-12A may be utilized for in vivo expansion of Tregs ａnd Bregs cells ａnd autologous Tregs ａnd Bregs cell immunotherapy.

蛋白编号：P29459 (R23-S219) & P29460 (I23-S328)

基因 ID：3592&3593

产品分类：细胞因子和生长因子  |  白细胞介素  |  白细胞介素-12  |  白介素-12A  |  细胞因子和生长因子  |  白细胞介素  |  白细胞介素-12  |  白介素-12B

Categories：Cytokines ａnd Growth Factors  |  Interleukin & Receptors  |  IL-12  |  IL-12 alpha  |  Cytokines ａnd Growth Factors  |  Interleukin & Receptors  |  IL-12  |  IL-12 beta

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