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Tris Buffered Saline

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MCE 站：Tris Buffered Saline

产品活性：Tris Buffered Saline 是分子生物学中常见的缓冲试剂，由 Tris 和 NaCl 组成。Tris Buffered Saline 可以调节 pH 和离子强度，被广泛应用于 DNA/RNA 提取、PCR 和蛋白质电泳。Tris Buffered Saline 可以纯化和保护核酸物质、可提高反应效率和浓度。在蛋白质电泳中，Tris Buffered Saline 可以稳定凝胶并维持 pH 值。总体而言，Tris Buffered Saline 在分子生物学应用中具有多功能性、良好溶解度和低毒性。

研究领域：Others

作用靶点：Biochemical Assay Reagents

In Vitro: Tris Buffered Saline is a commonly used buffered salt for molecular biology experiments, which is composed of trimethylmethacrylate (Tris) ａnd potash (NaCl). It has special features such as adjusting pH value ａnd ionic strength, high solubility ａnd low toxicity, ａnd is widely used in experiments such as DNA/RNA extraction, PCR, ａnd protein electrophoresis.
In DNA/RNA extraction experiments, Tris Buffered Saline is usually used for DNA/RNA purification, accelerating the reaction process ａnd balancing to determine the DNA/RNA structure. Tris Buffered Saline can effectively protect DNA/RNA from degradation by deoxyribonuclease (DNase) ａnd ribonuclease (RNase).
In PCR experiments. Tris Buffered Saline can be used in the preparation of PCR reaction systems.
Tris Buffered Saline can also be used as a gel rinse to maintain gel stability ａnd pH of the electrophoresis solution.

Tris Buffered Saline can be used in the following studies:
Immunogold-silver staining (IGSS) method to study immunoglobulins in reactive human tonsils.
Studying aggregation of purified samples of α-synuclein.
As a buffer for Western blot analysis.
Time-resolved fluorescence immunoassay.
As a buffer for light microscopy immunohistochemistry.

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