MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

TUNEL 细胞凋亡检测试剂盒 (Biotin)

MCE 站：TUNEL 细胞凋亡检测试剂盒 (Biotin)

MCE TUNEL 细胞凋亡检测试剂盒 (Biotin) 可用于检测培养细胞的凋亡情况，也可检测冷冻或石蜡切片中的细胞凋亡情况。

Description & Advantages:

Apoptosis is a form of programmed cell death. Based on the cellular changes during programmed cell death, apoptosis is often classified into early, middle, ａnd later stages. The later stages of apoptosis are characterized by changes in nuclear morphology, chromatin condensation, nuclear envelope degradation, ａnd DNA fragmentation.

The principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) in detecting cell apoptosis is that during apoptosis, certain DNA endonucleases are activated, which cleave genomic DNA between nucleosomes. In normal or proliferating cells, there is almost no DNA breakage, thus 3＇-OH formation is scarce, leading to minimal labeling. The exposed 3＇-OH can be catalyzed by terminal deoxynucleotidyl transferase (TdT) to incorporate biotin-labeled dUTP, which can conjugate with horseradish peroxidase (HRP) -labeled streptavidin-HRP. Through DAB staining catalyzed by HRP, apoptotic cells can be detected, enabling cell apoptosis detection.

MCE TUNEL Apoptosis Detection Kit (Biotin) can be used to detect apoptosis in cultured cells, as well as in frozen or paraffin sections. It selectively detects apoptotic cells ａnd distinguishes between apoptotic ａnd necrotic cells. Due to the distinct patterns of DNA fragmentation, necrotic cells or non-apoptotic cells with broken DNA strands caused by irradiation ａnd drug therapy will not be identified as apoptotic cells.

品牌介绍:
•   MCE (MedChemExpress) 拥有200 多种全球独家化合物库，我们致力于为全球科研客户提供前沿*全的高品质小分子活性化合物；
•   50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；
•   产品种类涵盖各种重组蛋白，多肽，常用试剂盒 ，更有 PROTAC、ADC 等特色产品，广泛应用于新药研发、生命科学等科研项目；
•   提供虚拟筛选，离子通道筛选，代谢组学分析检测分析，药物筛选等专业技术服务；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种期刊及制药收录了MCE客户的科研成果；
•   专业团队跟踪*新的制药及生命科学研究进展，为您提供全球*新的活性化合物；
•   与世界各大制药公司及知名科研机构建立了长期的合作。