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小鼠肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)ELISA试剂盒*上海新闻
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产品名称:小鼠肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)elisa试剂盒*上海新闻
英文名称:Mouse Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT
检测方法:ELISA
检测类型:酶免免疫夹心法
检测范围:见说明书
检测目标:人/动物
样品形式:血清/血浆/细胞培养上清/其它生物液体
保存与运输:2-8℃
应用范围:科研用检测试剂
小鼠肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)ELISA试剂盒*上海新闻样本处理及要求:
1. 血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2. 血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3. 尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4. 细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5. 组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8 ℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6. 标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
7. 不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
今品化学技术有限公司主要从事免疫学、分子生物学和常规生化试剂的研发、销售。并代理销售R&D、Sigma、Amresco 、Omega、Gibco、Pharmacia、HyClone、等多个国外知名品牌,致力于为广大高校、科研院所和企事业单位提供一流的科研试剂和完善的技术服务,满足生物化学、分子生物学 、细胞生物学、免疫学等生物科技实验需求。
小鼠肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)ELISA试剂盒*上海新闻相关产品发布:
Keratin 20 Antibody | P35900 | 49kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 20 Antibody | P35900 | 49kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 20 Antibody | P35900 | 49kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 20 Antibody | P35900 | 49kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 5 Antibody | P13647 | 62kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 5 Antibody | P13647 | 62kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 5 Antibody | P13647 | 62kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 5 Antibody | P13647 | 62kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 5 Antibody | P13647 | 62 kDa | Rabbit | Human,Mouse,Rat | WB,IHC |
Keratin 5 Antibody | P13647 | 62 kDa | Rabbit | Human,Mouse,Rat | WB,IHC |
Keratin 5 Antibody | P13647 | 62 kDa | Rabbit | Human,Mouse,Rat | WB,IHC |
Keratin 5 Antibody | P13647 | 62 kDa | Rabbit | Human,Mouse,Rat | WB,IHC |
Keratin 7 Antibody | P08729 | 51kDa | Rabbit | Human | WB IHC IF/ICC |
Keratin 7 Antibody | P08729 | 51kDa | Rabbit | Human | WB IHC IF/ICC |
Keratin 7 Antibody | P08729 | 51kDa | Rabbit | Human | WB IHC IF/ICC |
Keratin 7 Antibody | P08729 | 51kDa | Rabbit | Human | WB IHC IF/ICC |
Keratin 8 Antibody | P05787 | 55kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 8 Antibody | P05787 | 55kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 8 Antibody | P05787 | 55kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 8 Antibody | P05787 | 55kDa | Rabbit | Human, Mouse, Rat | WB IHC |
Keratin 8 Antibody | P05787 | 53kDa | Rabbit | Human, Mouse, Rat | WB IHC IF/ICC |
Keratin 8 Antibody | P05787 | 53kDa | Rabbit | Human, Mouse, Rat | WB IHC IF/ICC |
Keratin 8 Antibody | P05787 | 53kDa | Rabbit | Human, Mouse, Rat | WB IHC IF/ICC |
Keratin 8 Antibody | P05787 | 53kDa | Rabbit | Human, Mouse, Rat | WB IHC IF/ICC |
Ki67 Antibody | P46013 | 358kDa | Rabbit | Human, | IHC IF/ICC |
Ki67 Antibody | P46013 | 358kDa | Rabbit | Human, | IHC IF/ICC |
Ki67 Antibody | P46013 | 358kDa | Rabbit | Human, | IHC IF/ICC |
Ki67 Antibody | P46013 | 358kDa | Rabbit | Human, | IHC IF/ICC |
Ki67 Antibody | P46013 | Rabbit | Human | IHC,IF | |
Ki67 Antibody | P46013 | Rabbit | Human | IHC,IF | |
Ki67 Antibody | P46013 | Rabbit | Human | IHC,IF | |
Ki67 Antibody | P46013 | Rabbit | Human | IHC,IF | |
Ki67 Antibody | P46013 | 358kDa | Rabbit | Human | WB,IHC |
Ki67 Antibody | P46013 | 358kDa | Rabbit | Human | WB,IHC |
Ki67 Antibody | P46013 | 358kDa | Rabbit | Human | WB,IHC |
Ki67 Antibody | P46013 | 358kDa | Rabbit | Human | WB,IHC |
Kir6.2 Antibody | Q14654 | 44 kDa | Rabbit | Human,Mouse | WB,IHC,IF |
Kir6.2 Antibody | Q14654 | 44 kDa | Rabbit | Human,Mouse | WB,IHC,IF |
Kir6.2 Antibody | Q14654 | 44 kDa | Rabbit | Human,Mouse | WB,IHC,IF |
Kir6.2 Antibody | Q14654 | 44 kDa | Rabbit | Human,Mouse | WB,IHC,IF |
KIT Antibody | P10721 | 145kDa | Rabbit | Human, Mouse | WB 1:500-1:2000 |
KIT Antibody | P10721 | 145kDa | Rabbit | Human, Mouse | WB 1:500-1:2000 |
KIT Antibody | P10721 | 145kDa | Rabbit | Human, Mouse | WB 1:500-1:2000 |
KIT Antibody | P10721 | 145kDa | Rabbit | Human, Mouse | WB 1:500-1:2000 |
KITH_EBV Antibody | P03177 | 67kDa | Rabbit | Human, | WB |
KITH_EBV Antibody | P03177 | 67kDa | Rabbit | Human, | WB |
KITH_EBV Antibody | P03177 | 67kDa | Rabbit | Human, | WB |
KITH_EBV Antibody | P03177 | 67kDa | Rabbit | Human, | WB |
KLF11 Antibody | O14901 | 55kDa | Rabbit | Human, Mouse | WB |
KLF11 Antibody | O14901 | 55kDa | Rabbit | Human, Mouse | WB |
KLF11 Antibody | O14901 | 55kDa | Rabbit | Human, Mouse | WB |
KLF11 Antibody | O14901 | 55kDa | Rabbit | Human, Mouse | WB |
KLK3 Antibody | P07288 | 34kDa | Rabbit | Human | WB IHC |
KLK3 Antibody | P07288 | 34kDa | Rabbit | Human | WB IHC |
KLK3 Antibody | P07288 | 34kDa | Rabbit | Human | WB IHC |
KLK3 Antibody | P07288 | 34kDa | Rabbit | Human | WB IHC |
KSR2 Antibody | Q6VAB6 | 108kDa | Rabbit | Human, Mouse, | WB IF/ICC |
KSR2 Antibody | Q6VAB6 | 108kDa | Rabbit | Human, Mouse, | WB IF/ICC |
KSR2 Antibody | Q6VAB6 | 108kDa | Rabbit | Human, Mouse, | WB IF/ICC |
KSR2 Antibody | Q6VAB6 | 108kDa | Rabbit | Human, Mouse, | WB IF/ICC |
Ku70 Antibody | P12956 | 70kDa | Rabbit | Human, Mouse, Rat | WB IHC IF/ICC |
Ku70 Antibody | P12956 | 70kDa | Rabbit | Human, Mouse, Rat | WB IHC IF/ICC |
Ku70 Antibody | P12956 | 70kDa | Rabbit | Human, Mouse, Rat | WB IHC IF/ICC |
Ku70 Antibody | P12956 | 70kDa | Rabbit | Human, Mouse, Rat | WB IHC IF/ICC |
Ku70 Antibody | P12956 | 70kDa | Rabbit | Human, Mouse | WB IHC |
Ku70 Antibody | P12956 | 70kDa | Rabbit | Human, Mouse | WB IHC |
Ku70 Antibody | P12956 | 70kDa | Rabbit | Human, Mouse | WB IHC |
Ku70 Antibody | P12956 | 70kDa | Rabbit | Human, Mouse | WB IHC |
Ku70/80 Antibody | P12956/P13010 | 70, 82 kDa | Rabbit | Human,Mouse,Rat | WB,IHC,IF |
Ku70/80 Antibody | P12956/P13010 | 70, 82 kDa | Rabbit | Human,Mouse,Rat | WB,IHC,IF |
Ku70/80 Antibody | P12956/P13010 | 70, 82 kDa | Rabbit | Human,Mouse,Rat | WB,IHC,IF |
Ku70/80 Antibody | P12956/P13010 | 70, 82 kDa | Rabbit | Human,Mouse,Rat | WB,IHC,IF |