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小鼠骨成型蛋白2(BMP-2)ELISA试剂盒*上海新闻
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小鼠骨成型蛋白2(BMP-2)elisa试剂盒*上海新闻用途:本产品仅供科学研究
规格:48T/96T(凡购买本公司试剂盒提供免费代测服务)
Elisa试剂盒报价,Elisa试剂盒价格,Elisa试剂盒说明书,Elisa试剂盒技术,Elisa试剂盒售后,Elisa试剂盒代测详情咨询
试剂盒性能:
1.准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。
2.灵敏度:检测浓度小于10 pg/ml。
3.特异性:不与其它可溶性结构类似物交叉反应。
4.重复性:板内、板间变异系数均小于15%。
贮藏:2-8℃,避光防潮保存。
有效期:6个月
小鼠骨成型蛋白2(BMP-2)ELISA试剂盒*上海新闻操作步骤
1. 从室温平衡20min 后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。
2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;
3. 待测样本孔先加生物素10μL,再加待测样本50μL;
4. 随后标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。
5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5 次(也可用洗板机洗板)。
6. 每孔加入底物A、B 各50μL,37℃避光孵育15min。
7. 每孔加入终止液50μL,15min 内,在450nm 波长处测定各孔的OD 值。
洗板方法
1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min 后甩尽孔内液体,在吸水纸上拍干,如此洗板5 次。
2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5 次。
结果判断
绘制标准曲线:在Excel 工作表中,以标准品浓度作横坐标,对应OD 值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。
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小鼠骨成型蛋白2(BMP-2)ELISA试剂盒*上海新闻相关产品推荐:
CEP27 Antibody | Q9NVX0 | 27 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CEP27 Antibody | Q9NVX0 | 27 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CEP41 Antibody | Q9BYV8 | 41 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CEP41 Antibody | Q9BYV8 | 41 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CEP63 Antibody | Q96MT8 | 81 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CEP63 Antibody | Q96MT8 | 81 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CEP68 Antibody | Q76N32 | 81 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CEP68 Antibody | Q76N32 | 81 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CEP72 Antibody | Q9P209 | 72 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CEP72 Antibody | Q9P209 | 72 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CSPP1 Antibody | Q1MSJ5 | 146 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CSPP1 Antibody | Q1MSJ5 | 146 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CEP250 Antibody | Q9BV73 | 281kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CEP250 Antibody | Q9BV73 | 281kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CDR1 Antibody | P51861 | 31 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CDR1 Antibody | P51861 | 31 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CP Antibody | P00450 | 122kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CP Antibody | P00450 | 122kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
PDE2A Antibody | O00408 | 106 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
PDE2A Antibody | O00408 | 106 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
PDE3A Antibody | Q14432 | 125 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
PDE3A Antibody | Q14432 | 125 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
PDE3B Antibody | Q13370 | 124kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
PDE3B Antibody | Q13370 | 124kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
PDE5A Antibody | O76074 | 100 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
PDE5A Antibody | O76074 | 100 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CKLF Antibody | Q9UBR5 | 17 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CKLF Antibody | Q9UBR5 | 17 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CLCN2 Antibody | P51788 | 98 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CLCN2 Antibody | P51788 | 98 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CLCNKB Antibody | P51801 | 75kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CLCNKB Antibody | P51801 | 75kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
CLCN1 Antibody | P35523 | 109kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CLCN1 Antibody | P35523 | 109kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CHSY3 Antibody | Q70JA7 | 100 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CHSY3 Antibody | Q70JA7 | 100 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
SLC18A1 Antibody | P54219 | 56 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
SLC18A1 Antibody | P54219 | 56 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CBX2 Antibody | Q14781 | 56 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CBX2 Antibody | Q14781 | 56 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CHD2 Antibody | O14647 | 211kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
CHD2 Antibody | O14647 | 211kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CHD6 Antibody | Q8TD26 | 305kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
CHD6 Antibody | Q8TD26 | 305kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CHD8 Antibody | Q9HCK8 | 290kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CHD8 Antibody | Q9HCK8 | 290kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CHD9 Antibody | Q3L8U1 | 326kDa | Rabbit | Human, Mouse | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CHD9 Antibody | Q3L8U1 | 326kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
KIF4B Antibody | Q2VIQ3 | 140kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
KIF4B Antibody | Q2VIQ3 | 140kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
LUC7L3 Antibody | O95232 | 51 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
LUC7L3 Antibody | O95232 | 51 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CMTM5 Antibody | Q96DZ9 | 25 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CMTM5 Antibody | Q96DZ9 | 25 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CKLF7 Antibody | Q96FZ5 | 19 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CKLF7 Antibody | Q96FZ5 | 19 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CKLF8 Antibody | Q8IZV2 | 20 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CKLF8 Antibody | Q8IZV2 | 20 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CLDN12 Antibody | P56749 | 27 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CLDN12 Antibody | P56749 | 27 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
CLDN14 Antibody | O95500 | 26 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CLDN14 Antibody | O95500 | 26 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CLDN18 Antibody | P56856 | 28 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CLDN18 Antibody | P56856 | 28 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CLDN20 Antibody | P56880 | 24 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
CLDN20 Antibody | P56880 | 24 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
CLDN22/24 Antibody | Q8N7P3/A6NM45 | 24 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CLDN22/24 Antibody | Q8N7P3/A6NM45 | 24 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CLDN23 Antibody | Q96B33 | 32 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CLDN23 Antibody | Q96B33 | 32 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CLDN9 Antibody | O95484 | 23 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CLDN9 Antibody | O95484 | 23 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CPSF6 Antibody | Q16630 | 59 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
CPSF6 Antibody | Q16630 | 59 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CLASP2 Antibody | O75122 | 141kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CLASP2 Antibody | O75122 | 141kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
DENND4A Antibody | Q7Z401 | 209kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
DENND4A Antibody | Q7Z401 | 209kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
F11 Antibody | P03951 | 70 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
F11 Antibody | P03951 | 70 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
F13B Antibody | P05160 | 76kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
F13B Antibody | P05160 | 76kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
COPG Antibody | Q9Y678 | 98 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
COPG Antibody | Q9Y678 | 98 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
CCDC109A Antibody | Q8NE86 | 40 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CCDC109A Antibody | Q8NE86 | 40 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CCDC131 Antibody | O60293 | 226 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CCDC131 Antibody | O60293 | 226 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CCDC8 Antibody | Q9H0W5 | 59 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CCDC8 Antibody | Q9H0W5 | 59 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
C1QTNF8 Antibody | P60827 | 28 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
C1QTNF8 Antibody | P60827 | 28 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
C1QTNF9B Antibody | B2RNN3 | 35 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
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