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小鼠活化素A(ACV-A)ELISA试剂盒*上海新闻
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详细内容
小鼠活化素A(ACV-A)elisa试剂盒*上海新闻样品收集、处理及保存方法
1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000 转离心10 分钟将血清和红细胞迅速小心地分离。
2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000 转离心30 分钟取上清。
3. 细胞上清液:3000 转离心10 分钟去除颗粒和聚合物。
4. 组织匀浆:将组织加入适量生理盐水捣碎。3000 转离心10 分钟取上清。
5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。
自备物品
1. 酶标仪(450nm)
2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL
3. 37℃恒温箱
小鼠活化素A(ACV-A)ELISA试剂盒*上海新闻操作注意事项
1. 试剂盒保存在2-8℃,使用前室温平衡20 分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。
2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。
3. 浓度为0 的S0 号标准品即可视为阴性对照或者空白;预处理后的样本无需稀释,直接取50μL 加样即可。
4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。
5. 所有液体组分使用前充分摇匀。
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小鼠活化素A(ACV-A)ELISA试剂盒*上海新闻相关产品推荐:
C1QL4 Antibody | Q86Z23 | 25 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
C1QL4 Antibody | Q86Z23 | 25 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
C7 Antibody | P10643 | 94kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
C7 Antibody | P10643 | 94kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CFD Antibody | P00746 | 27 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CFD Antibody | P00746 | 27 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CR1 Antibody | P17927 | 224kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CR1 Antibody | P17927 | 224kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
NCAPD3 Antibody | P42695 | 169kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
NCAPD3 Antibody | P42695 | 169kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CNTN3 Antibody | Q9P232 | 113kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CNTN3 Antibody | Q9P232 | 113kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
CNTNAP1 Antibody | P78357 | 156kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CNTNAP1 Antibody | P78357 | 156kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CNTNAP3 Antibody | Q9BZ76 | 141kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CNTNAP3 Antibody | Q9BZ76 | 141kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CNTNAP4 Antibody | Q9C0A0 | 145 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CNTNAP4 Antibody | Q9C0A0 | 145 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
HSD11B2 Antibody | P80365 | 44 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
HSD11B2 Antibody | P80365 | 44 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CNGA3 Antibody | Q16281 | 79 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CNGA3 Antibody | Q16281 | 79 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CNGB3 Antibody | Q9NQW8 | 92 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
CNGB3 Antibody | Q9NQW8 | 92 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CTH Antibody | P32929 | 45 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
CTH Antibody | P32929 | 45 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
CST8 Antibody | O60676 | 16 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
CST8 Antibody | O60676 | 16 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
CST9L2 Antibody | Q5W188 | 17 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CST9L2 Antibody | Q5W188 | 17 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
CST5 Antibody | P28325 | 16 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
CST5 Antibody | P28325 | 16 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
CST7 Antibody | O76096 | 16 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CST7 Antibody | O76096 | 16 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CST6 Antibody | Q15828 | 17 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
CST6 Antibody | Q15828 | 17 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
DYNC1I2 Antibody | Q13409 | 71 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
DYNC1I2 Antibody | Q13409 | 71 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
CNDP2 Antibody | Q96KP4 | 53kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
CNDP2 Antibody | Q96KP4 | 53kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
PLA2G4B Antibody | P0C869 | 88 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
PLA2G4B Antibody | P0C869 | 88 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
PLA2G4F Antibody | Q68DD2 | 95 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
PLA2G4F Antibody | Q68DD2 | 95 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
NT5C2 Antibody | P49902 | 65 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
NT5C2 Antibody | P49902 | 65 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
DOCK11 Antibody | Q5JSL3 | 238kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
DOCK11 Antibody | Q5JSL3 | 238kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
DOCK7 Antibody | Q96N67 | 242kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
DOCK7 Antibody | Q96N67 | 242kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
DHRS1 Antibody | Q96LJ7 | 34 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
DHRS1 Antibody | Q96LJ7 | 34 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
DHRS10 Antibody | Q9BPX1 | 28 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
DHRS10 Antibody | Q9BPX1 | 28 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
DHRS11 Antibody | Q6UWP2 | 28 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
DHRS11 Antibody | Q6UWP2 | 28 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
DHRS7 Antibody | Q9Y394 | 38 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
DHRS7 Antibody | Q9Y394 | 38 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
DHRS9 Antibody | Q9BPW9 | 35 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
DHRS9 Antibody | Q9BPW9 | 35 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |