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小鼠可溶性肌球蛋白重链1(sMHC-1)ELISA试剂盒*精品推荐
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小鼠可溶性肌球蛋白重链1(sMHC-1)elisa试剂盒*精品推荐操作注意事项
1. 试剂应按标签说明书储存,使用前恢复到室温。稀稀过后的标准品应丢弃,不可保存。
2. 实验中不用的板条应立即放回包装袋中,密封保存,以免变质。
3. 不用的其它试剂应包装好或盖好。不同批号的试剂不要混用。保质前使用。
4. 使用一次性的吸头以免交叉污染,吸取终止液和底物A、B液时,避免使用带金属部分的加样器。
5. 使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。
6. 洗涤酶标板时应充分拍干,不要将吸水纸直接放入酶标反应孔中吸水。
7. 底物A应挥发,避免长时间打开盖子。底物B对光敏感,避免长时间暴露于光下。避免用手接触,有毒。实验完成后应立即读取OD值。
8. 加入试剂的顺序应一致,以保证所有反应板孔温育的时间一样。
9. 按照说明书中标明的时间、加液的量及顺序进行温育操作。
小鼠可溶性肌球蛋白重链1(sMHC-1)ELISA试剂盒*精品推荐样品收集、处理及保存方法
1、 血清-----操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后,1000×g离心10分钟将血清和红细胞迅速小心地分离。
2、 血浆-----EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3、 细胞上清液---1000×g离心10分钟去除颗粒和聚合物。
4、 组织匀浆-----将组织加入适量生理盐水捣碎。1000×g离心10分钟,取上清液
5、 保存------如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
小鼠可溶性肌球蛋白重链1(sMHC-1)ELISA试剂盒*精品推荐相关产品发布:
ARHGEF4 Antibody | Q9NR80 | 79 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
ARHGEF6 Antibody | Q15052 | 87kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
ARHGEF6 Antibody | Q15052 | 87kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
RHOF Antibody | Q9HBH0 | 24 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
RHOF Antibody | Q9HBH0 | 24 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
RND2 Antibody | P52198 | 25 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
RND2 Antibody | P52198 | 25 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
RTKN Antibody | Q9BST9 | 63 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
RTKN Antibody | Q9BST9 | 63 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
RHOXF1 Antibody | Q8NHV9 | 21 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
RHOXF1 Antibody | Q8NHV9 | 21 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
RPL22/L1 Antibody | Q6P5R6/P35268 | 15 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
RPL22/L1 Antibody | Q6P5R6/P35268 | 15 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
RPS6KL1 Antibody | Q9Y6S9 | 60 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
RPS6KL1 Antibody | Q9Y6S9 | 60 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
BMS1 Antibody | Q14692 | 146 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
BMS1 Antibody | Q14692 | 146 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
SERP1 Antibody | Q9Y6X1 | 7 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
SERP1 Antibody | Q9Y6X1 | 7 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
RPAP1 Antibody | Q9BWH6 | 153 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
RPAP1 Antibody | Q9BWH6 | 153 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
RPAP3 Antibody | Q9H6T3 | 76kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
RPAP3 Antibody | Q9H6T3 | 76kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
CROCC Antibody | Q5TZA2 | 229 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
CROCC Antibody | Q5TZA2 | 229 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
ROBO1 Antibody | Q9Y6N7 | 181 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
ROBO1 Antibody | Q9Y6N7 | 181 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
SCARA3 Antibody | Q6AZY7 | 65 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
SCARA3 Antibody | Q6AZY7 | 65 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
SFRP3 Antibody | Q92765 | 36 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
SFRP3 Antibody | Q92765 | 36 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
SEPT11 Antibody | Q9NVA2 | 49 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
SEPT11 Antibody | Q9NVA2 | 49 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
SEPT5 Antibody | Q99719 | 43 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
SEPT5 Antibody | Q99719 | 43 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
SRR Antibody | Q9GZT4 | 37 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
SRR Antibody | Q9GZT4 | 37 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
STK25 Antibody | O00506 | 48 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
STK25 Antibody | O00506 | 48 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
STK31 Antibody | Q9BXU1 | 116kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
STK31 Antibody | Q9BXU1 | 116kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |
STK38L Antibody | Q9Y2H1 | 54 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
STK38L Antibody | Q9Y2H1 | 54 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
NEK4 Antibody | P51957 | 95 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 50ug/50ul |
NEK4 Antibody | P51957 | 95 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
PIM2 Antibody | Q9P1W9 | 34 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
PIM2 Antibody | Q9P1W9 | 34 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
RIOK1 Antibody | Q9BRS2 | 66 kDa | Rabbit | Human, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
RIOK1 Antibody | Q9BRS2 | 66 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
SRPK2 Antibody | P78362 | 78 kDa | Rabbit | Human, Rat | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
SRPK2 Antibody | P78362 | 78 kDa | Rabbit | Human | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
ULK2 Antibody | Q8IYT8 | 113kDa | Rabbit | Human, Mouse | WB 1:1000-3000 IHC 1:200 | 50ug/50ul |
ULK2 Antibody | Q8IYT8 | 113kDa | Rabbit | Human, Mouse | WB 1:1000-3000 IHC 1:200 | 100ug/100ul |
ULK4 Antibody | Q96C45 | 142 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
ULK4 Antibody | Q96C45 | 142 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
VRK1 Antibody | Q99986 | 45 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 50ug/50ul |
VRK1 Antibody | Q99986 | 45 kDa | Rabbit | Human, Mouse | WB 1:1000-3000 | 100ug/100ul |
PPP4R1 Antibody | Q8TF05 | 107 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
PPP4R1 Antibody | Q8TF05 | 107 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
SERPINB10 Antibody | P48595 | 45 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
SERPINB10 Antibody | P48595 | 45 kDa | Rabbit | Human | WB 1:1000-3000 | 100ug/100ul |
SERPINB6 Antibody | P35237 | 43 kDa | Rabbit | Human | WB 1:1000-3000 | 50ug/50ul |
SERPINB6 Antibody | P35237 | 43 kDa | Rabbit | Human, Mouse, Rat | WB 1:1000-3000 | 100ug/100ul |