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小鼠基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)ELISA试剂盒*今品化学新闻
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品牌名称：$brandModel.Title(进口品牌)型号：48T/96T 原产地：中国大陆 发布时间：2018/5/16 14:00:57更新时间：2024/12/26 11:35:41
产品摘要：小鼠基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)ELISA试剂盒*今品化学新闻为今品化学技术（上海）有限公司主打产品，质量好,发货及时,灵敏度高，特异性强，重复性好，其他动物类试剂盒种属一应俱全,指标数千种。公司配有专业实验室可寄样免费代检测支持现场观摩,提供售前售中售后全方位服务，欢迎来电询价或在线咨询索取产品说明书~
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详细内容
小鼠基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)elisa试剂盒*今品化学新闻使用目的：
为定量（性）测定活化的目标浓度的血清，血浆，组织匀浆，细胞培养上清及其他生物液体,仅用于科研,不得用于医学诊断。
实验原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被目标物抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的目标物呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。
小鼠基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)ELISA试剂盒*今品化学新闻组成
名称 96孔配置 48孔配置 备注
微孔酶标板 12孔×8条 12孔×4条 无
标准品 0.5mL*6管 0.5mL*6管 按说明书复溶
样本稀释液 10mL 10mL 无
检测抗体-HRP 10mL 5mL 无
20×洗涤缓冲液 25mL 15mL 按说明书稀释
底物A 6mL 3mL 无
底物B 6mL 3mL 无
终止液 6mL 3mL 无
封板膜 2张 2张 无
说明书 1份 1份 无
自封袋 1个 1个 无
小鼠基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)ELISA试剂盒*今品化学新闻样本处理及要求
1. 血清：全血标本请于室温放置2小时或4℃过夜后于2000g离心20分钟，取上清即可检测，或将标本放于-20℃或-80℃保存，但应避免反复冻融。
2. 血浆：可用EDTA或肝素作为抗凝剂，标本采集后30分钟内于2 - 8℃ 2000g离心30分钟，或将标本放于-20℃或-80℃保存，但应避免反复冻融
3. 细胞培养物上清或其它生物标本：2000g离心20分钟，取上清即可检测，或将标本放于-20℃或-80℃保存，但应避免反复冻融。
注：标本溶血会影响*后检测结果，因此溶血标本不宜进行此项检测。
需要而未提供的试剂和器材
1. 酶标仪（450nm）
2. 高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL
3. 37℃恒温箱
4. 蒸馏水或去离子水
注意事项
1. 严格按照规定的时间和温度进行温育以保证准确结果。所有试剂都必须在使用前达到室温20-25℃。使用后立即冷藏保存试剂。
2. 洗板不正确可以导致不准确的结果。在加入底物前确保尽量吸干孔内液体。温育过程中不要让微孔干燥掉。
3. 消除板底残留的液体和手指印，否则影响OD值。
4. 底物显色液应呈无色或很浅的颜色，已经变蓝的底物液不能使用。
5. 避免试剂和标本的交叉污染以免造成错误结果。
6. 在储存和温育时避免强光直接照射。
7. 平衡至室温后再打开密封袋以防水滴凝聚在冷板条上。
8. 任何反应试剂不能接触漂白溶剂或漂白溶剂所散发的强烈气体。任何漂白成分都会破坏试剂盒中反应试剂的生物活性。
9. 不能使用过期产品。
10. 如果可能传播疾病，所有的样品都应管理好，按照规定的程序处理样品和检测装置。
试剂准备
试剂盒从冷藏环境中取出应在室温平衡后方可使用。
1. 标准品复溶：试剂盒提供6管标准品，每管已标定浓度，并且冻干。实验前在每个标准品管中加入0.5mL样本稀释液，盖好后静置10分钟以上，然后反复颠倒/搓动助其溶解，使其恢复为每个标准品管身标注的浓度。
2. 20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份20×洗涤缓冲液加19份蒸馏水。
小鼠基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)ELISA试剂盒*今品化学新闻操作步骤
1. 从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。
2. 设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；
3. 样本孔中加入待测样本50μL；空白孔不加。
4. 除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。
5. 弃去液体，吸水纸上拍干，每孔加满洗涤液（350μL），静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板4-5次（也可用洗板机洗板）。
6. 每孔加入底物A、B各50μL，37℃避光孵育15min。
7. 每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。
实验结果计算
以所测标准品的OD值为横坐标，标准品的浓度值为纵坐标，在坐标纸上或用相关软件绘制标准曲线，并得到直线回归方程，将样品的OD值
| 100μg | MBD3 Antibody | O95983 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
| 100μg | Calsenilin/KCNIP3 (Ab-63) Antibody | Q9Y2W7 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | Calsenilin/KCNIP3 (Phospho-Ser63) Antibody | Q9Y2W7 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | RECK Antibody | O95980 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | TBX15/18 Antibody | Q96SF7/O95935 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | MEKKK 4 Antibody | O95819 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | ZNF682 Antibody | O95780 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | GAS41 Antibody | O95619 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
| 100μg | CBX6 Antibody | O95503 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | SIX6 Antibody | O95475 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | ZIC1/2/3/4/5 Antibody | Q15915/O95409/O60481/Q8N9L1/Q96T25 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | TNFRSF6B Antibody | O95407 | Human (Identities = 100%, Positives = 100%) | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | MED26 Antibody | O95402 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | MAP3K6 Antibody | O95382 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 85%, Positives = 85%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | TFIP8 Antibody | O95379 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | TRIM16 Antibody | O95361 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | NDUC2 Antibody | O95298 | Human (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg | MYST2 Antibody | O95251 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | AurB (Ab-232) Antibody | Q96GD4 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | AurB (Phospho-Thr232) Antibody | Q96GD4 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | LRAT Antibody | O95237 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | ZNF265 Antibody | O95218 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | TNFSF15 Antibody | O95150 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | ALX3 Antibody | O95076 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | HEXIM1 Antibody | O94992 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | APC1 Antibody | Q9H1A4 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | APC1 (Phospho-Ser688) Antibody | Q9H1A4 | Human, Mouse | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | JHD3B Antibody | O94953 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | FIR Antibody | O94887 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | SNAPC5 Antibody | O75971 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
| 1mg/ml | AKAP3 Antibody | O75969 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | FSHR Antibody | P23945 | Human (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | CDKA2 Antibody | O75956 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | DGKI Antibody | O75912 | Human, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
| 1mg/ml | TUSC2 Antibody | O75896 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:1000 | |
| 100μg | TALL-2 Antibody | O75888 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | CIB2 Antibody | O75838 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:5000 | |
| 100μg | BCAR3 Antibody | O75815 | Human | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg | Caper Antibody | O75787 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | FOXH1 Antibody | O75593 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | TAF5L Antibody | O75529 | Human | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg | SUPT3H Antibody | O75486 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | ADA2L Antibody | O75478 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg | Vinculin (Ab-821) Antibody | Q64727 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | Vinculin (Phospho-Tyr821) Antibody | Q64727 | Human, Mouse, Rat | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | MED24 Antibody | O75448 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | Maf Antibody | O75444 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg | DNA Polymerase θ Antibody | O75417 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:20000 | |
| 100μg | TACC1 Antibody | O75410 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | LAT3 Antibody | O75387 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | TRIM3 Antibody | O75382 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | TBX10 Antibody | O75333 | Human | WB: 1:500~1:1000 ELISA: 1:5000 | |
| 100μg | SIN3B Antibody | O75182 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | PFDN1 Antibody | O60925 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | Dyskerin Antibody | O60832 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | PFKFB2 Antibody | O60825 | Human | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | VEGFR2 Antibody | P35968 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | VEGFR2 (Phospho-Tyr1054) Antibody | P35968 | Human, Mouse, Rat | IHC: 1:50~1:100 ELISA: 1:40000 | |
| 100μg | Musculin Antibody | O60682 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg | LMX1B Antibody | O60663 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:40000 | |
| 100μg | BUB1B Antibody | O60566 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | SDCG1 Antibody | O60524 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:20000 | |
| 100μg | hnRNP Q Antibody | O60506 | Human, Mouse, Rat | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | ZIC1/2/3 Antibody | Q15915 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 | |
| 100μg | DLX3 Antibody | O60479 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg | HBP1 Antibody | O60381 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg | ZEB2 Antibody | O60315 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg | MAST3 Antibody | O60307 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg | ZNF387 Antibody | O60284 | Human | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg | Tryptophan Hydroxylase (Ab-260) Antibody | P17752 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | Tryptophan Hydroxylase (Phospho-Ser260) Antibody | P17752 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 1mg/ml | DNA Polymerase ζ Antibody | O60673 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:40000 | |
| 1mg/ml | CTRO Antibody | O14578 | Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| 1mg/ml | ERK8 (Ab-175/177) Antibody | Q8TD08 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000 | |
| 1mg/ml | EGFR (Phospho-Tyr1016) Antibody | P00533 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 | |
| 100μg | MED14 Antibody | O60244 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | EFNA2 Antibody | O43921 | Human, Mouse | WB: 1:500~1:1000 ELISA: 1:5000 | |
| 100μg | AIRE Antibody | O43918 | Human, Mouse | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000 | |
| 100μg | AKAP8 Antibody | O43823 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | Tryptophan Hydroxylase (Ab-58) Antibody | P17752 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | Tryptophan Hydroxylase (Phospho-Ser58) Antibody | P17752 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 | |
| 100μg | BUB1 Antibody | O43683 | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 ELISA: 1:10000 | |
| 100μg | HAIR Antibody | O43593 | Human (Identities = 100%, Positives = 100%) | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 | |
| 100μg | AKAP10 Antibody | O43572 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg | Syndecan4 (Ab-179) Antibody | P31431 | Human, Mouse, Rat | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 | |
| 100μg | TNF14 Antibody | O43557 | Human (Identities = 100%, Positives = 100%) | IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | TNF12 Antibody | O43508 | Human, Mouse | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | TBX1 Antibody | O43435 | Human | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000 | |
| 100μg | ANP32C Antibody | O43423 | Human | WB: 1:500~1:1000 ELISA: 1:10000 | |
| 100μg | TPD54 Antibody | O43399 | Human, Mouse, Rat | WB: 1:500~1:1000 ELISA: 1:10000 |
